Mohammed Amany Tharwat, Mohamed Amany Abdel-Rahman, Ali Haytham
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Acta Histochem. 2017 May;119(4):357-363. doi: 10.1016/j.acthis.2017.03.004. Epub 2017 Apr 2.
This study aimed to assess the potential apoptotic and oxidative damaging impacts of Triclosan (TCS) and Sodium Fluoride (NaF) administered separately or in combination, in rats for thirty days. For this purpose, forty immature female Sprague-Dawley rats were equally allocated into five groups. TCS group administered 185mg TCS/kg Bw dissolved in distilled water (DW), while its control group received only DW. NaF group was given 50mg/kg NaF in corn oil BW and its respective control had corn oil alone. The co-treated group was administered TCS and NaF. The oxidative stress biomarkers were evaluated in lung tissue homogenate and apoptotic proteins (BcL-2 and Caspase-3) expression were quantified in lung tissues. The results of TCS or NaF treated groups revealed a prominent depletion of super oxide dismutase (SOD), Catalase (CAT) and Glutathione (GSH) in lung tissue homogenate. On contrary, a marked increase in the tissue levels of Malondialdehyde (MDA) and lactate dehydrogenase (LDH) enzymatic assay levels. The co-exposed group evoked less severity in the oxidative stress biomarkers concentration than individually exposed groups. The apoptotic genes protein expression was significantly higher in TCS or NaF treated rats when compared to the control with intense to moderate immunolabeling of the bronchiolar lining epithelium and surrounding mononuclear inflammatory cells. On the contrary, no significant differences were detected in the expression of the investigated apoptotic biomarkers between the control and the combined exposed group. We concluded that the exposure to either TCS or NaF resulted in significant perturbations in lung tissue after short term oral administration at variable instances but the co-exposure resulted in less severe toxicological consequences.
本研究旨在评估单独或联合给予大鼠三氯生（TCS）和氟化钠（NaF）30天的潜在凋亡和氧化损伤影响。为此，将40只未成熟雌性Sprague-Dawley大鼠平均分为五组。TCS组给予溶解于蒸馏水（DW）中的185mg TCS/kg体重，而其对照组仅接受DW。NaF组给予50mg/kg NaF溶于玉米油中，其相应对照组仅给予玉米油。联合处理组给予TCS和NaF。评估肺组织匀浆中的氧化应激生物标志物，并对肺组织中凋亡蛋白（BcL-2和Caspase-3）的表达进行定量。TCS或NaF处理组的结果显示，肺组织匀浆中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）显著减少。相反，丙二醛（MDA）的组织水平和乳酸脱氢酶（LDH）酶活性测定水平显著增加。联合暴露组引起的氧化应激生物标志物浓度的严重程度低于单独暴露组。与对照组相比，TCS或NaF处理的大鼠中凋亡基因蛋白表达显著更高，细支气管内衬上皮和周围单核炎性细胞有强烈至中度的免疫标记。相反，对照组和联合暴露组之间在所研究的凋亡生物标志物的表达上未检测到显著差异。我们得出结论，短期口服给予TCS或NaF在不同情况下均导致肺组织的显著扰动，但联合暴露导致的毒理学后果较轻。
