Wu Xiaoling, Li Xin, Yang Miyi, Zeng Haozhe, Zhang Sanbing, Lu Runhua, Gao Haixiang, Xu Donghui
Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China.
Department of Applied Chemistry, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing 100193, China.
J Chromatogr A. 2017 May 12;1497:1-8. doi: 10.1016/j.chroma.2017.03.005. Epub 2017 Mar 6.
Phytophagous mites are usually considered a difficult problem for agricultural planting, and acaricides are applied to control diseases and pests. However, the overdose and misusage of acaricides causes pesticide residues. In this work, a simple and practical ionic liquid-based TiO nanofluid, effervescence-assisted, dispersive liquid-liquid microextraction (EA-DLLME) method was developed to detect acaricides in honey and tea by high-performance liquid chromatography (HPLC-DAD). Oleophilic TiO nanoparticles were synthesized by a facile solvothermal method to obtain greater stability of the nanofluid. The experimental parameters were optimized by a one-factor-at-a-time approach and included the effervescent tablet composition, ionic liquid selection, extractant composition, nanofluid volume, extraction temperature, extraction time and desorption conditions. Under the optimized conditions, the linear ranges of this proposed method were 0.5-500μgL, with correlation coefficients in the range of 0.9985-1.0000. The extraction efficiencies for the target analytes varied from 70.70 to 84.58%. The detection and quantitation limits were in the ranges of 0.04-0.18μgL and 0.13-0.60μgL, respectively. The intra- and inter-day relative standard deviations (n=3) were found to range from 2.32 to 5.71%, which showed perfect repeatability. Overall, the EA-DLLME method was time-saving and environmentally friendly, with future potential for microextraction.
植食性螨类通常被认为是农业种植中的一个难题，人们使用杀螨剂来控制病虫害。然而，杀螨剂的过量使用和误用会导致农药残留。在本研究中，开发了一种简单实用的基于离子液体的二氧化钛纳米流体、泡腾辅助分散液液微萃取（EA-DLLME）方法，用于通过高效液相色谱法（HPLC-DAD）检测蜂蜜和茶叶中的杀螨剂。通过简便的溶剂热法合成了亲油性二氧化钛纳米颗粒，以获得纳米流体更高的稳定性。通过单因素法优化了实验参数，包括泡腾片组成、离子液体选择、萃取剂组成、纳米流体体积、萃取温度、萃取时间和解吸条件。在优化条件下，该方法的线性范围为0.5-500μg/L，相关系数在0.9985-1.0000之间。目标分析物的萃取效率在70.70%至84.58%之间。检测限和定量限分别在0.04-0.18μg/L和0.13-0.60μg/L范围内。日内和日间相对标准偏差（n=3）在2.32%至5.71%之间，显示出良好的重复性。总体而言，EA-DLLME方法省时且环保，具有未来微萃取的潜力。
