Wu Pei, Liu Yang, Jiang Wei-Dan, Jiang Jun, Zhang Yong-An, Zhou Xiao-Qiu, Feng Lin
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, China.
Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, 611130, China.
Fish Shellfish Immunol. 2017 Jun;65:34-41. doi: 10.1016/j.fsi.2017.03.053. Epub 2017 Mar 30.
This study aimed to investigate the effects of choline deficiency on intestinal inflammation of fish after Aeromonas hydrophila infection and the potential molecular mechanisms. Juvenile Jian carp (Cyprinus carpio var. Jian) were fed two diets containing choline at 165 (deficient group) and 607 mg/kg diet respectively for 65 days. Choline deficiency decreased intestinal lysozyme activity, C3 and IgM contents, increased acid phosphatase activity, downregulated mRNA levels of antimicrobial peptides [liver-expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, hepcidin and defensin], cytokines [interleukin (IL) 6a, tumor necrosis factor α (TNF-α), interferon γ2b (IFN-γ2b), IL-6b and transforming growth factor β2 (TGF-β2) only in proximal intestine, IL-10 in mid and distal intestine], immune-related signaling molecules [Toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), nuclear factor kappa B (NF-κB), inhibitor of NF-κB (IκB), Janus kinase 3 (JAK3), and signal transducers and activators of transcription 5 (STAT5)], tight junction proteins (claudin 3b, claudin 3c, claudin 11 and occludin), and mitogen-activated protein kinases p38 (p38) in proximal and distal intestine of juvenile Jian carp after A. hydrophila challenge. In contrast, choline deficiency upregulated mRNA levels of antimicrobial peptides (LEAP-2A, LEAP-2B, hepcidin and defensin), cytokines (IL-6b, IFN-γ2b and TGF-β2), immune-related signaling molecules (TLR4, MyD88, NF-κB, IκB, JAK3, STAT4 in three intestinal segments, and STAT6), claudin 11, and p38 in mid intestine of fish. This study provides new finding that choline deficiency-induced immune responses against A. hydrophila infection were varied among three intestinal segments in fish.
本研究旨在探讨胆碱缺乏对嗜水气单胞菌感染后鱼类肠道炎症的影响及其潜在分子机制。将幼建鲤（Cyprinus carpio var. Jian）分别投喂两种胆碱含量为165（缺乏组）和607 mg/kg饲料的日粮，持续65天。胆碱缺乏降低了肠道溶菌酶活性、C3和IgM含量，增加了酸性磷酸酶活性，下调了抗菌肽[肝脏表达抗菌肽（LEAP）2A、LEAP - 2B、铁调素和防御素]、细胞因子[白细胞介素（IL）6a、肿瘤坏死因子α（TNF - α）、干扰素γ2b（IFN - γ2b）、IL - 6b和仅在近端肠道中的转化生长因子β2（TGF - β2）、中肠和远端肠道中的IL - 10]、免疫相关信号分子[Toll样受体4（TLR4）、髓样分化初级反应88（MyD88）、核因子κB（NF - κB）、NF - κB抑制剂（IκB）、Janus激酶3（JAK3）和信号转导子和转录激活子5（STAT5）]、紧密连接蛋白（claudin 3b、claudin 3c、claudin 11和闭合蛋白）以及嗜水气单胞菌攻击后幼建鲤近端和远端肠道中的丝裂原活化蛋白激酶p38（p38）的mRNA水平。相反，胆碱缺乏上调了鱼类中肠抗菌肽（LEAP - 2A, LEAP - 2B, 铁调素和防御素）、细胞因子（IL - 6b、IFN - γ2b和TGF - β2）、免疫相关信号分子（三个肠段中的TLR4、MyD88、NF - κB、IκB、JAK3、STAT4和STAT6）、claudin 11和p38的mRNA水平。本研究提供了新的发现，即胆碱缺乏诱导的针对嗜水气单胞菌感染的免疫反应在鱼类的三个肠段中存在差异。
