Dreiling C E, Norrgran C, Smith E
Division of Biochemistry, School of Medicine, University of Nevada, Reno 89557.
Metab Pediatr Syst Ophthalmol (1985). 1985;8(2-3):69-74.
2',3'-Cyclic nucleotide 3'-phosphohydrolase (CNPase) activity is reduced in myelin during multiple sclerosis. Therefore, we have investigated whether the enzyme is also depressed in non-neural membranes during this disease. MS and control erythrocyte membranes and whole lymphocytes were assayed for CNPase activity. The MS erythrocyte membrane preparations were found to exhibit up to 45% lower CNPase levels than the controls (P less than 0.02). There was no significant difference in the lymphocyte CNPase levels in MS and control groups. When control erythrocyte membrane preparations were assayed in the presence of MS plasma they were inhibited 100% by MS (but not by control) plasma. These data suggest that erythrocyte membrane CNPase activity is significantly reduced in MS patients and that the reduction in activity may be due to an inhibitor present in MS plasma.
在多发性硬化症中，髓鞘内的2',3'-环核苷酸3'-磷酸水解酶（CNPase）活性降低。因此，我们研究了在这种疾病中该酶在非神经膜中是否也受到抑制。对多发性硬化症患者和对照者的红细胞膜及全淋巴细胞进行了CNPase活性检测。发现多发性硬化症患者的红细胞膜制剂中CNPase水平比对照组低达45%（P<0.02）。多发性硬化症组和对照组的淋巴细胞CNPase水平无显著差异。当在多发性硬化症患者血浆存在的情况下检测对照红细胞膜制剂时，它们被多发性硬化症患者（而非对照者）的血浆100%抑制。这些数据表明，多发性硬化症患者的红细胞膜CNPase活性显著降低，且活性降低可能是由于多发性硬化症患者血浆中存在的一种抑制剂所致。
