Fuellgraf Hannah, Schilling Oliver, Lai Zon Weng, Kulemann Birte, Timme Sylvia, Makowiec Frank, Shahinian Jasmin H, Hoeppner Jens, Werner Martin, Hopt Ulrich T, Wellner Ulrich F, Bronsert Peter
Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany;; German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany;; Institute of Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany;; BIOSS Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany.
J Cancer. 2017 Feb 25;8(4):657-664. doi: 10.7150/jca.16200. eCollection 2017.
Carcinoembryonic antigen cell adhesion molecule (CEA) is a commonly immunohistochemically used antibody in pathological routine diagnostics with an overexpression in different cancers. We aimed to examine the immunohistochemically detectable CEA level in ampullary cancer and to correlate it with clinico-pathological data. Shot-gun proteomics revealed CEA in undifferentiated ampullary cancer cell lines. Next, tumor tissue of 40 ampullary cancers of a retrospective single center cohort of 40 patients was stained immunohistochemically for CEA; CEA expression was determined and correlated with clinico-pathological data. Thirty-six patient specimens were included in statistical analysis. CEA expression and lymph node ratio (LNR) were the only independent predictors of overall survival in multivariate analysis. To our knowledge, cell line and patient cohorts are the largest and characterized cohorts examined for CEA so far. Hereby, CEA expression in ampullary cancer cells permits an estimation of outcome and suggests an opportunity for individualized CEA-directed therapy. Further trials with larger cohorts are needed to verify our results and to integrate CEA immunohistochemistry into clinical routine.
癌胚抗原细胞粘附分子（CEA）是病理常规诊断中常用的免疫组化抗体，在不同癌症中存在过表达。我们旨在检测壶腹癌中免疫组化可检测到的CEA水平，并将其与临床病理数据相关联。鸟枪法蛋白质组学在未分化的壶腹癌细胞系中发现了CEA。接下来，对一个40例患者的回顾性单中心队列中的40例壶腹癌肿瘤组织进行CEA免疫组化染色；确定CEA表达并将其与临床病理数据相关联。36例患者标本纳入统计分析。在多变量分析中，CEA表达和淋巴结比率（LNR）是总生存的唯一独立预测因素。据我们所知，细胞系和患者队列是迄今为止检测CEA的最大且具有特征的队列。因此，壶腹癌细胞中的CEA表达有助于评估预后，并提示个体化CEA导向治疗的机会。需要更大队列的进一步试验来验证我们的结果，并将CEA免疫组化纳入临床常规。
