公牛繁殖力及其与密度梯度筛选精子的关系。
Bull Fertility and Its Relation with Density Gradient Selected Sperm.
作者信息
Allouche Lynda, Madani Toufik, Mechmeche Mohamed, Clement Laetitia, Bouchemal Allaoua
机构信息
Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, Ferhat Abbas Setif 1 University, Setif, Algeria.
Department of Agronomy, Faculty of Nature and Life Sciences, Ferhat Abbas Setif 1 University, Setif, Algeria.
出版信息
Int J Fertil Steril. 2017 Apr-Jun;11(1):55-62. doi: 10.22074/ijfs.2016.4721. Epub 2016 Nov 11.
BACKGROUND
Sperm selection method is usually used to collect these cells for -assisted reproduction. Few studies reported the relationship of fertility and semen parameters after sperm selection; hence, the present study attempted to assess different semen parameters after post-thaw or sperm selection, using density gradient separation BoviPure®, to predict fertility.
MATERIALS AND METHODS
In this experimental study, frozen semen quality of four Montbeliarde bulls were assessed after post-thaw (PT) or after sperm selection (SSp), using density gradient separation BoviPure®, to predict the fertility rate in vivo. In addition to PT or SSp, semen was examined for concentration, motility, morphology abnormalities, viability, acrosome and plasma membrane integrities. Fertility was measured as non-return rates within 56 days after the first insemination (NRR) or as corrected NRR, expressed as CNRR, to the factors influencing fertility using linear mixed model. Non-parametric Kruskal-Wallis test was performed to compare semen parameter variables. Fertility rates were compared using Chi-square test. Pearson correlation analysis was used to test the relationship between CNRR and semen parameters. Data was analysed using SPSS package program, version 21.0.
RESULTS
Most of the examined bulls exhibited a high fertility rate (3/4 bulls, 62.1- 81.8% for NRR or 67.2-98.5% for CNRR). Fertility rate, expressed as CNRR, was significantly related to semen parameters after SSp, but not after PT. Thus, CNRR was increased with decrease of total motility, progressive spermatozoa and abaxial implantation frequencies after SSp (r=-0.999, P=0.001; r=-0.990, P=0.010; r=-0.988, P= 0.012, respectively); while, CNRR was decreased with decrease of SSp immotile spermatozoa (r=+0.995, P=0.005), underlying that maximal limit of determined immotile spermatozoa is 47%.
CONCLUSION
High frequencies of total and progressive motility spermatozoa, and abaxial implantation in gradient selected sperm appear to be not favorable for fertility .
背景
精子筛选方法通常用于收集用于辅助生殖的细胞。很少有研究报道精子筛选后生育力与精液参数之间的关系；因此，本研究试图使用密度梯度分离法BoviPure®评估解冻后或精子筛选后的不同精液参数，以预测生育力。
材料与方法
在本实验研究中，对4头蒙贝利亚公牛的冷冻精液质量在解冻后（PT）或精子筛选后（SSp）进行评估，使用密度梯度分离法BoviPure®，以预测体内的生育率。除了PT或SSp外，还对精液的浓度、活力、形态异常、存活率、顶体和质膜完整性进行了检查。生育力通过首次输精后56天内的未返情率（NRR）或校正后的NRR（表示为CNRR）来衡量，使用线性混合模型对影响生育力的因素进行校正。进行非参数Kruskal-Wallis检验以比较精液参数变量。使用卡方检验比较生育率。使用Pearson相关分析来检验CNRR与精液参数之间的关系。使用SPSS软件包程序21.0版对数据进行分析。
结果
大多数被检查的公牛表现出较高的生育率（3/4头公牛，NRR为62.1 - 81.8%，或CNRR为67.2 - 98.5%）。以CNRR表示的生育率在SSp后与精液参数显著相关，但在PT后则不然。因此，在SSp后，随着总活力、渐进性精子和背侧着床频率的降低，CNRR升高（r = -0.999，P = 0.001；r = -0.990，P = 0.010；r = -0.988，P = 0.012）；而随着SSp中不活动精子的减少，CNRR降低（r = +0.995，P = 0.005），这表明确定的不活动精子的最大极限为47%。
结论
梯度选择精子中总活力和渐进性活力精子的高频率以及背侧着床似乎对生育力不利。
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