Shiozawa M, Ochiya T, Hatada I, Imamura T, Okudaira Y, Hiraoka H, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Oncogene. 1988 May;2(5):523-6.
The lca-transforming DNA was isolated from human hepatocellular carcinomas. This gene has no homology with known transforming DNA from human sources, and its role in neoplastic tissue formation has been left unanswered. In this communication, we report that RNAs prepared from human fetal livers hybridize to the lca DNA probe. The RNA is 1.8 kilobase in size and appears in the fetal liver only for a limited period during its development, viz. 19 weeks through 24 weeks of gestation. No other tissues carry detectable levels of the lca messenger RNA. Fetal hepatocytes at 5 weeks of gestation showed no transcripts of lca, but upon culturing for 2 more weeks in vitro, the cells became producers of the lca messenger RNA. These results suggest that the lca plays some role in the proliferative stage of the liver.
LCA转化DNA是从人类肝细胞癌中分离出来的。该基因与已知的人类来源的转化DNA没有同源性，其在肿瘤组织形成中的作用尚未得到解答。在本通讯中，我们报告从人类胎儿肝脏制备的RNA与LCA DNA探针杂交。该RNA大小为1.8千碱基，仅在胎儿肝脏发育的有限时期出现，即妊娠19周 至24周。没有其他组织携带可检测水平的LCA信使RNA。妊娠5周的胎儿肝细胞未显示LCA转录本，但在体外再培养2周后，这些细胞成为LCA信使RNA的产生者。这些结果表明LCA在肝脏的增殖阶段起某种作用。
