一氧化氮抑制花生（Arachis hypogaea L.）根尖铝诱导的细胞程序性死亡。
Nitric oxide inhibits aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips.
机构信息
College of Agronomy, Guangxi University, Nanning 530004, PR China; Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, PR China.
College of Agronomy, Guangxi University, Nanning 530004, PR China.
出版信息
J Hazard Mater. 2017 Jul 5;333:285-292. doi: 10.1016/j.jhazmat.2017.03.049. Epub 2017 Mar 24.
It had been reported that Aluminum (Al) stress altered nitric oxide (NO) concentration and induced programmed cell death (PCD) in plants. However, the relationship between NO and PCD occurrence under Al stress is unclear. The results showed that cell death induced by Al was significant negative correlation with the inhibition of Al on root elongation growth in peanut. AlCl at 100μmolL induced DNA ladder, chromatin condensation, typical apoptotic chromatin condensation staining with DAPI, apoptosis related gene Hrs203j expression and caspase3-like protease activation in peanut root tip cells, and showed that Al-induced cell death in peanut root tip cells was a typical PCD. Exogenous NO donor sodium nitroprusside (SNP) at 200μmolL inhibited Al-induced PCD occurrence, but NO specific scavenger cPTIO aggravated PCD production. It suggests that NO is a negative regulator of Al-induced PCD in peanut root tips.
据报道，铝（Al）胁迫会改变一氧化氮（NO）浓度并诱导植物程序性细胞死亡（PCD）。然而，在 Al 胁迫下 NO 与 PCD 发生的关系尚不清楚。结果表明，Al 诱导的细胞死亡与 Al 对花生根伸长生长的抑制呈显著负相关。100μmolL 的 AlCl 诱导 DNA 梯状带，染色质凝聚，用 DAPI 进行典型的凋亡染色质凝聚染色，花生根尖细胞中凋亡相关基因 Hrs203j 的表达和半胱天冬酶 3 样蛋白酶的激活，表明 Al 诱导的花生根尖细胞死亡是一种典型的 PCD。200μmolL 的外源一氧化氮供体硝普钠（SNP）抑制了 Al 诱导的 PCD 的发生，但一氧化氮特异性清除剂 cPTIO 加重了 PCD 的产生。这表明 NO 是花生根尖中 Al 诱导的 PCD 的负调节剂。
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