National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, PR China; Guangxi Key Laboratory for Agro-Environment and Agro-Product Safety, Nanning 530004, PR China.
National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, PR China.
Gene. 2021 May 20;781:145535. doi: 10.1016/j.gene.2021.145535. Epub 2021 Feb 23.
Aluminum (Al) toxicity is an important factor in limiting peanut growth on acidic soil. The molecular mechanisms underlying peanut responses to Al stress are largely unknown. In this study, we performed transcriptome analysis of the root tips (0-1 cm) of peanut cultivar ZH2 (Al-sensitive) and 99-1507 (Al-tolerant) respectively. Root tips of peanuts that treated with 100 μM Al for 8 h and 24 h were analyzed by RNA-Seq, and a total of 8,587 differentially expressed genes (DEGs) were identified. GO and KEGG pathway analysis excavated a group of important Al-responsive genes related to organic acid transport, metal cation transport, transcription regulation and programmed cell death (PCD). These homologs were promising targets to modulate Al tolerance in peanuts. It was found that the rapid transcriptomic response to Al stress in 99-1507 helped to activate effective Al tolerance mechanisms. Protein and protein interaction analysis indicated that MAPK signal transduction played important roles in the early response to Al stress in peanuts. Moreover, weighted correlation network analysis (WGCNA) identified a predicted EIL (EIN3-like) gene with greatly increased expression as an Al-associated gene, and revealed a link between ethylene signaling transduction and Al resistance related genes in peanut, which suggested the enhanced signal transduction mediated the rapid transcriptomic responses. Our results revealed key pathways and genes associated with Al stress, and improved the understanding of Al response in peanut.
铝（Al）毒性是限制花生在酸性土壤中生长的一个重要因素。花生对铝胁迫响应的分子机制在很大程度上尚不清楚。在这项研究中，我们分别对花生品种 ZH2（铝敏感型）和 99-1507（铝耐受型）的根尖（0-1cm）进行了转录组分析。用 100μM Al 处理花生根尖 8h 和 24h 后，通过 RNA-Seq 分析，共鉴定出 8587 个差异表达基因（DEGs）。GO 和 KEGG 通路分析挖掘了一组与有机酸转运、金属阳离子转运、转录调控和细胞程序性死亡（PCD）相关的重要 Al 响应基因。这些同源物是调节花生耐铝性的有前途的靶标。研究发现，99-1507 对铝胁迫的快速转录组响应有助于激活有效的耐铝机制。蛋白质和蛋白质相互作用分析表明，MAPK 信号转导在花生对铝胁迫的早期响应中发挥重要作用。此外，加权相关网络分析（WGCNA）鉴定出一个预测的 EIL（EIN3 样）基因，其表达显著增加，作为一个与 Al 相关的基因，并揭示了花生中乙烯信号转导与 Al 抗性相关基因之间的联系，这表明增强的信号转导介导了快速的转录组响应。我们的研究结果揭示了与铝胁迫相关的关键途径和基因，提高了对花生中铝响应的认识。
