神经生长因子可短暂增加嗜铬细胞瘤PC12h细胞的四氢生物蝶呤和总生物蝶呤含量。
Nerve growth factor transiently increases tetrahydrobiopterin and total biopterin contents of pheochromocytoma PC12h cells.
作者信息
Suzuki H, Nakanishi N, Yamada S
机构信息
Department of Pedodontics, Josai Dental University, Saitama, Japan.
出版信息
Biochem Biophys Res Commun. 1988 May 31;153(1):382-7. doi: 10.1016/s0006-291x(88)81235-x.
Nerve growth factor (NGF) is known to induce differentiation of pheochromocytoma into sympathetic neuron-like cells. Tetrahydrobiopterin (BPH4) and total biopterin (BP) levels in PC12h, a subclonal line of PC12, were transiently increased by NGF: the increase in BPH4 and BP reached the maximum (20-25 ng/mg protein = about 2-fold over the control level) at 24 h after the treatment was started. After 2-3 days, the BPH4 and BP levels decreased to the same level as in control cells. The NGF concentration which gave a half maximal BP increase by 24 h-treatment was around 1 ng/ml.
已知神经生长因子（NGF）可诱导嗜铬细胞瘤分化为交感神经元样细胞。PC12的亚克隆系PC12h中的四氢生物蝶呤（BPH4）和总生物蝶呤（BP）水平可被NGF短暂升高：开始处理后24小时，BPH4和BP的升高达到最大值（20 - 25 ng/mg蛋白质 = 约为对照水平的2倍）。2 - 3天后，BPH4和BP水平降至与对照细胞相同的水平。经24小时处理使BP增加至最大值一半的NGF浓度约为1 ng/ml。
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