Salvia-Trujillo L, Verkempinck S H E, Sun L, Van Loey A M, Grauwet T, Hendrickx M E
Laboratory of Food Technology, Leuven Food Science and Nutrition Research Centre (LFoRCe), Department of Microbial and Molecular Systems (M(2)S), KU Leuven, Kasteelpark Arenberg 22, PB 2457, 3001 Leuven, Belgium.
Food Chem. 2017 Aug 15;229:653-662. doi: 10.1016/j.foodchem.2017.02.146. Epub 2017 Mar 1.
Carotenoid-enriched oil-in-water emulsions with different droplet sizes (small: d 0.72μm; medium: d 1.9μm; large: d 15.1μm) were subjected to simulated gastrointestinal conditions. The kinetics of lipolysis, micelle formation and carotenoid bioaccessibility were monitored during the intestinal phase. The rates of all three processes increased with decreasing droplet size. The large droplet size emulsion contained undigested oil at the end of digestion, whereas an almost complete hydrolysis was observed for the other two emulsions. The sub-micron emulsion presented a higher conversion of MAGs to FFAs during digestion, which led to a higher concentration of FFAs in the mixed micelles. The incorporation of carotenoids into mixed micelles occurred faster and reached a higher final value for the small droplet size emulsion, leading to final carotenoids bioaccessibility values of around 70%. This work provides valuable information for developing in silico models to simulate the lipid digestibility and carotenoid bioaccessibility.
对具有不同液滴尺寸（小：d 0.72μm；中：d 1.9μm；大：d 15.1μm）的富含类胡萝卜素的水包油乳液进行模拟胃肠道条件处理。在肠道阶段监测脂肪分解、胶束形成和类胡萝卜素生物可及性的动力学。所有这三个过程的速率都随着液滴尺寸的减小而增加。大液滴尺寸乳液在消化结束时含有未消化的油，而其他两种乳液观察到几乎完全水解。亚微米乳液在消化过程中呈现出更高的甘油单酯向游离脂肪酸的转化率，这导致混合胶束中游离脂肪酸的浓度更高。类胡萝卜素掺入混合胶束的过程对小液滴尺寸乳液来说发生得更快且最终值更高，导致最终类胡萝卜素生物可及性值约为70%。这项工作为开发计算机模型以模拟脂质消化率和类胡萝卜素生物可及性提供了有价值的信息。
