Henrionnet Christel, Dumas Dominique, Hupont Sébastien, Stoltz Jean François, Mainard Didier, Gillet Pierre, Pinzano Astrid
Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS - Université de Lorraine, Bâtiment Biopôle, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
Plateforme d'Imagerie Cellulaire PTIBC-IBISA, FR3209 CNRS, Faculté de Médecine, 9 avenue de la Forêt de Haye, BP 50184, F54505 Vandoeuvre-lès-Nancy, France.
Biomed Mater Eng. 2017;28(s1):S229-S235. doi: 10.3233/BME-171645.
In tissue engineering approaches, the quality of substitutes is a key element to determine its ability to treat cartilage defects. However, in clinical practice, the evaluation of tissue-engineered cartilage substitute quality is not possible due to the invasiveness of the standard procedure, which is to date histology. The aim of this work was to validate a new innovative system performed from two-photon excitation laser adapted to an optical macroscope to evaluate at macroscopic scale the collagen network in cartilage tissue-engineered substitutes in confrontation with gold standard histologic techniques or immunohistochemistry to visualize type II collagen. This system permitted to differentiate the quality of collagen network between ITS and TGF-β1 treatments. Multiscale large field imaging combined to multimodality approaches (SHG-TCSPC) at macroscopical scale represent an innovative and non-invasive technique to monitor the quality of collagen network in cartilage tissue-engineered substitutes before in vivo implantation.
