Amity School of Earth and Environmental Science, Amity University Gurgaon, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Bioresour Technol. 2017 Oct;242:2-6. doi: 10.1016/j.biortech.2017.03.124. Epub 2017 Mar 24.
Carbon dioxide utilizing bacterium Bacillus sp. SS105 was isolated from FACE (free air CO enriched) sample. The strain was grown in shake flask containing minimal salt medium with 50mM NaHCO as autotrophic carbon source and molasses as a low cost byproduct for mixotrophic growth. Carbon dioxide sequestration property of Bacillus sp. SS105 was determined by enzyme assay of carbonic anhydrase and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO). Along with CO sequestration this strain produced biosurfactant and its characterization by FTIR and H NMR indicated lipopeptide nature. Optimization of process parameter along with nutrient sources for higher biosurfactant production was done by Response Surface Methodology (RSM). Under optimized conditions, the yield of biosurfactant and biomass was 2.65 and 2.78gL respectively. The study revealed simultaneous CO sequestration and biosurfactant production by Bacillus sp. SS105.
从 FACE（自由空气 CO2 富集）样本中分离出一种利用二氧化碳的细菌，即芽孢杆菌 SS105 菌株。该菌株在含有 50mM NaHCO3 的最小盐培养基中进行摇瓶培养，以 NaHCO3 作为自养碳源，糖蜜作为混合营养生长的低成本副产物。通过碳酸酐酶和核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）的酶活性测定来确定芽孢杆菌 SS105 的二氧化碳固定性能。除了二氧化碳固定外，该菌株还产生生物表面活性剂，其结构特征通过傅里叶变换红外光谱（FTIR）和核磁共振（H NMR）分析表明是脂肽类物质。通过响应面法（RSM）对更高生物表面活性剂产量的工艺参数和营养源进行了优化。在优化条件下，生物表面活性剂和生物量的产量分别为 2.65 和 2.78gL。该研究揭示了芽孢杆菌 SS105 同时进行 CO2 固定和生物表面活性剂生产。
