von Knebel Doeberitz M, Oltersdorf T, Schwarz E, Gissmann L
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Cancer Res. 1988 Jul 1;48(13):3780-6.
In cervical carcinomas and cell lines derived from these tumors the DNA of specific types of human papilloma viruses (HPVs) is integrated into the host cell genome. The two viral open reading frames E6 and E7 are consistently transcribed in tumors and cell lines, and the respective proteins were detected in cells cultured in vitro. As shown here, modulation of HPV 18 E6 and E7 gene expression in C4-1 cervical carcinoma cells is accompanied by an altered cell growth. HPV 18 E6 and E7 expression can be enhanced by glucocorticoid treatment of C4-1 cells, and an increased cell proliferation is observed. In contrast, after introduction of complementary RNA to the HPV 18 E6 and E7 open reading frames, their expression is inhibited, and decreased cell growth is observed. These results support the hypothesis that expression of HPV E6 and E7 open reading frames is directly involved in growth regulation of cervical carcinoma cells.
在宫颈癌及源自这些肿瘤的细胞系中，特定类型的人乳头瘤病毒（HPV）的DNA整合到宿主细胞基因组中。病毒的两个开放阅读框E6和E7在肿瘤和细胞系中持续转录，并且在体外培养的细胞中检测到了相应的蛋白质。如下所示，C4-1宫颈癌细胞中HPV 18 E6和E7基因表达的调节伴随着细胞生长的改变。通过对C4-1细胞进行糖皮质激素处理可增强HPV 18 E6和E7的表达，并且观察到细胞增殖增加。相反，在引入针对HPV 18 E6和E7开放阅读框的互补RNA后，它们的表达受到抑制，并且观察到细胞生长减缓。这些结果支持了HPV E6和E7开放阅读框的表达直接参与宫颈癌细胞生长调节的假说。
