Jang Ho Hee, Lee Hae Na, Kim So-Young, Hong Suntaek, Lee Won-Suk
Department of Biochemistry, College of Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon Univerisity, Incheon, Republic of Korea.
Department of Surgery, Gil Medical Center, Gachon University, Incheon, Republic of Korea.
Anticancer Res. 2017 Apr;37(4):1779-1785. doi: 10.21873/anticanres.11511.
Recent studies have shown a potential role of RNA-binding proteins (RBPs) in a variety of biological pathways, including cancer progression, whilst their expression in various tumor types may be associated with patient prognosis. However, the role of the RBP family members has not been explored in colon cancer and their possible use as prognostic biomarkers is largely unknown.
To determine the prognostic role of three RBP genes: insulin-like growth factor-binding protein 2 (IGFBP2), RNA-binding motif protein 3 (RBM3), and cold-inducible RNA-binding protein (CIRP) in colon cancer.
We examined the RNA expression of IGFBP2, RBM3, and CIRP in 94 human colon cancer samples along with matched normal tissue samples from each patient using quantitative real-time polymerase chain reaction (qRT-PCR). No significant associations were observed between RNA expression of RBPs and the studied clinical features. The estimated 5-year disease-free survival rate was significantly better for patients with higher expression of RBM3 and CIRP, while patient survival was not significantly correlated to IGFBP2 expression.
RBM3 and CIRP may be useful prognostic biomarkers of colon cancer.
最近的研究表明，RNA结合蛋白（RBPs）在包括癌症进展在内的多种生物学途径中具有潜在作用，而它们在各种肿瘤类型中的表达可能与患者预后相关。然而，RBP家族成员在结肠癌中的作用尚未得到探索，其作为预后生物标志物的潜在用途也大多未知。
为了确定三种RBP基因：胰岛素样生长因子结合蛋白2（IGFBP2）、RNA结合基序蛋白3（RBM3）和冷诱导RNA结合蛋白（CIRP）在结肠癌中的预后作用。
我们使用定量实时聚合酶链反应（qRT-PCR）检测了94例人类结肠癌样本以及来自每位患者的匹配正常组织样本中IGFBP2、RBM3和CIRP的RNA表达。未观察到RBPs的RNA表达与所研究的临床特征之间存在显著关联。RBM3和CIRP高表达的患者估计5年无病生存率显著更好，而患者生存率与IGFBP2表达无显著相关性。
RBM3和CIRP可能是有用的结肠癌预后生物标志物。
