Goodyear Shaun, Brinster Ralph
Cold Spring Harb Protoc. 2017 Apr 3;2017(4):pdb.prot094235. doi: 10.1101/pdb.prot094235.
Spermatogonial stem cells (SSCs) are located in the basal region of the seminiferous tubules and provide a self-renewing reservoir from which progenitor spermatogonia arise and transiently amplify in number before transition to a differentiating state. SSC transplantation involves grafting mouse testicular cells into the seminiferous tubules of an aspermic recipient testis that is generated either by chemical (e.g., busulfan) treatment or genetic mutations.
精原干细胞（SSCs）位于生精小管的基部区域，提供一个自我更新的细胞库，祖代精原细胞从中产生，并在转变为分化状态之前数量短暂增加。SSC移植涉及将小鼠睾丸细胞移植到无精子受体睾丸的生精小管中，该受体睾丸可通过化学（如白消安）处理或基因突变产生。
