Mustonen Anne-Mari J, Finnilä Mikko A J, Puukka Katri S, Jämsä Timo J, Saarakkala Simo, Tuukkanen Juha K, Nieminen T Petteri
University of Eastern Finland, Faculty of Health Sciences, School of Medicine, Institute of Biomedicine/Anatomy, PO Box 1627, Kuopio FI-70211, Finland
University of Eastern Finland, Faculty of Science and Forestry, Department of Environmental and Biological Sciences, PO Box 111, Joensuu FI-80101, Finland.
J Exp Biol. 2017 Jun 15;220(Pt 12):2196-2202. doi: 10.1242/jeb.135475. Epub 2017 Apr 3.
The raccoon dog () is a promising animal model capable of preventing disuse-induced osteoporosis. Previous data suggest that this species resembles bears in the preservation of bone mass and biomechanical properties during prolonged passivity and catabolism. This longitudinal study examined the osteological properties of tibiae in farm-bred raccoon dogs that were either fed or fasted (=6 per group) for a 10 week period. Peripheral quantitative computed tomography was utilized and plasma markers of bone turnover measured before fasting and at 9 weeks followed by mechanical testing (three-point bending), micro-computed tomography and Fourier transform infrared imaging at 10 weeks. Passive wintering with prolonged catabolism (body mass loss 32%) had no significant effects on bone mineralization, porosity or strength. The concentration of C-terminal telopeptide of type I collagen, indicative of bone resorption, increased in the plasma of the fasted raccoon dogs, while the bone formation markers were unchanged. The levels of 25-hydroxyvitamin D were reduced in the fasted animals. Based on these data, the preservation of bone in wintering raccoon dogs shares characteristics with that of bears with no apparent decrease in the formation of bone but increased resorption. To conclude, raccoon dogs were able to minimize bone loss during a 10 week period of catabolism and passivity.
貉是一种有潜力的动物模型，能够预防废用性骨质疏松。先前的数据表明，在长期的被动状态和分解代谢过程中，该物种在骨量和生物力学特性的保持方面与熊相似。这项纵向研究检查了农场饲养的貉的胫骨的骨学特性，这些貉被分为喂食组或禁食组（每组6只），为期10周。利用外周定量计算机断层扫描技术，在禁食前和第9周测量骨转换的血浆标志物，然后在第10周进行力学测试（三点弯曲）、微计算机断层扫描和傅里叶变换红外成像。经历长期分解代谢（体重减轻32%）的被动越冬对骨矿化、孔隙率或强度没有显著影响。禁食的貉血浆中I型胶原C端肽浓度升高，这表明骨吸收增加，而骨形成标志物没有变化。禁食动物的25-羟基维生素D水平降低。基于这些数据，越冬貉的骨骼保存与熊的骨骼保存具有共同特征，即骨形成没有明显减少，但骨吸收增加。总之，貉能够在10周的分解代谢和被动状态期间将骨质流失降至最低。
