Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, China.
Center Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
Naunyn Schmiedebergs Arch Pharmacol. 2017 Jul;390(7):711-720. doi: 10.1007/s00210-017-1365-4. Epub 2017 Apr 3.
Reactive oxygen species (ROS) is a pivotal pathogenic factor in the development of osteoporosis. Dalbergioidin (DAL) can be isolated from Uraria crinite, an edible herb used as a natural food for childhood skeletal dysplasia. Recent research has implicated DAL as having an antiosteoporosis effect, although the mechanism of this is unclear. We used an effective oxidative stress model, induced by hydrogen peroxide (HO) in osteoblastic MC3T3-E1 cells, to investigate the protective effects of DAL in osteoporosis and the underlying molecular mechanisms. The results indicated that treatment with DAL maintained redox balance, reduced MC3T3-E1 cell apoptosis, improved alkaline phosphatase activity, and elevated the osteogenic-related protein expression of Runx2, Osterix, and BMP2 against oxidative damage induced by HO. The potential molecular mechanism involved in the protective effect of DAL against HO-induced cell death in MC3T3-E1 cells may lie in the activation of the PI3K/AKT/SMAD1 cell signal pathway. Taken together, the results indicated that the potential protective effects of DAL against osteoporosis were linked to a reduction in oxidative damage, suggesting that DAL may be useful in bone metabolism diseases, particularly osteoporosis.
活性氧（ROS）是骨质疏松症发展的关键致病因素。槐定碱（DAL）可从食用草药豨莶中分离出来，用于治疗儿童骨骼发育不良。最近的研究表明 DAL 具有抗骨质疏松作用，但具体机制尚不清楚。我们使用过氧化氢（HO）诱导的成骨细胞 MC3T3-E1 细胞的有效氧化应激模型，研究 DAL 在骨质疏松症中的保护作用及其潜在的分子机制。结果表明，DAL 处理维持了氧化还原平衡，减少了 MC3T3-E1 细胞凋亡，提高了碱性磷酸酶活性，并上调了 Runx2、Osterix 和 BMP2 等成骨相关蛋白的表达，从而减轻了 HO 诱导的氧化损伤。DAL 对 MC3T3-E1 细胞 HO 诱导细胞死亡的保护作用的潜在分子机制可能涉及 PI3K/AKT/SMAD1 细胞信号通路的激活。综上所述，DAL 对骨质疏松症的潜在保护作用与减轻氧化损伤有关，提示 DAL 可能对骨骼代谢疾病，尤其是骨质疏松症具有治疗作用。
