Lee Sojin, Jin Seon-Pil, Kim Yeon Kyung, Sung Gun Yong, Chung Jin Ho, Sung Jong Hwan
Department of Chemical Engineering, Hongik University, Seoul, Republic of Korea.
Department of Dermatology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Biomed Microdevices. 2017 Jun;19(2):22. doi: 10.1007/s10544-017-0156-5.
Current in vitro skin models do not recapitulate the complex architecture and functions of the skin tissue. In particular, on-chip construction of an in vitro model comprising the epidermis and dermis layer with vascular structure for mass transport has not been reported yet. In this study, we aim to develop a microfluidic, three-dimensional (3D) skin chip with fluidic channels using PDMS and hydrogels. Mass transport within the collagen hydrogel matrix was verified with fluorescent model molecules, and a transport-reaction model of oxygen and glucose inside the skin chip was developed to aid the design of the microfluidic skin chip. Comparison of viabilities of dermal fibroblasts and HaCaT cultured in the chip with various culture conditions revealed that the presence of flow plays a crucial role in maintaining the viability, and both cells were viable after 10 days of air exposure culture. Our 3D skin chip with vascular structures can be a valuable in vitro model for reproducing the interaction between different components of the skin tissue, and thus work as a more physiologically realistic platform for testing skin reaction to cosmetic products and drugs.
当前的体外皮肤模型无法重现皮肤组织的复杂结构和功能。特别是，尚未有关于构建包含具有用于物质运输的血管结构的表皮和真皮层的体外模型的片上构建的报道。在本研究中，我们旨在使用聚二甲基硅氧烷（PDMS）和水凝胶开发一种带有流体通道的微流控三维（3D）皮肤芯片。用荧光模型分子验证了胶原水凝胶基质内的物质运输，并建立了皮肤芯片内氧气和葡萄糖的运输反应模型，以辅助微流控皮肤芯片的设计。在不同培养条件下对芯片中培养的真皮成纤维细胞和HaCaT细胞活力的比较表明，流动的存在对维持活力起着关键作用，并且在空气暴露培养10天后两种细胞均存活。我们具有血管结构的3D皮肤芯片可以成为用于再现皮肤组织不同成分之间相互作用的有价值的体外模型，从而作为测试皮肤对化妆品和药物反应的更符合生理实际的平台。
