DJ-1和HE4在子宫内膜癌患者中的临床意义。

The clinical significance of DJ-1 and HE4 in patients with endometrial cancer.

作者信息

Benati Marco, Montagnana Martina, Danese Elisa, Paviati Elisa, Giudici Silvia, Ruzzenente Orazio, Franchi Massimo, Lippi Giuseppe

机构信息

Department of Neurological, Biomedical and Movement Sciences, Clinical Biochemistry Section, University of Verona, Verona, Italy.

Department of Surgery, Obstetrics and Gynaecology Section, University of Verona, Verona, Italy.

出版信息

J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22223. Epub 2017 Apr 4.

DOI:10.1002/jcla.22223

PMID:28374920

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6817134/

Abstract

BACKGROUND

The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic biomarker for breast cancer, melanoma, and pancreatic cancer. The aim of this study was to compare the diagnostic performances of DJ-1 and HE4 measured in EC patients and healthy controls (HC).

METHODS

Forty-five patients (63.9±12.0 years) with EC and 29 (63.2±13.3 years) HC were enrolled. Serum concentrations of DJ-1 and HE4 were measured using ELISA kits developed by R&D (Minneapolis, USA) and Fujirebio Diagnostic (Malvern, PA, USA), respectively. Differences between EC patients and HC were assessed by Mann-Whitney test and associations were tested by Spearman's correlation. The diagnostic performance was assessed using receiver operating characteristics (ROC) curves analysis.

RESULTS

Serum DJ-1 concentrations were found to be higher in EC patients than in HC (9533.6 vs 1988.5 pg/mL; P<.0001). The area under the ROC curve (ROC-AUC) was 0.95 (P<.0001). At the cut-off of 3654 pg/mL, the sensitivity and specificity were 0.89 and 0.90, respectively. HE4 serum levels were higher in EC patients than in HC (75.3 vs 56.2 pmol/L; P=.019), with an AUC of 0.66 (P=.020). The AUC obtained by the combination of the two markers resulted 0.96 (P<.0001).

CONCLUSION

These results suggest that increased serum DJ-1 levels are associated with EC and that this biomarker may be potentially useful for diagnosing EC.

摘要

背景

子宫内膜癌（EC）早期检测的非侵入性诊断方法仍然有限。迄今为止，人附睾蛋白4（HE4）已得到深入研究，但其在EC诊断中的作用存在争议。DJ-1是一种由癌细胞分泌的癌蛋白，最近被确定为乳腺癌、黑色素瘤和胰腺癌的潜在诊断生物标志物。本研究的目的是比较在EC患者和健康对照（HC）中测量的DJ-1和HE4的诊断性能。

方法

纳入45例（63.9±12.0岁）EC患者和29例（63.2±13.3岁）HC。分别使用美国明尼阿波利斯的R&D公司和美国宾夕法尼亚州马尔文的富士瑞必欧诊断公司开发的ELISA试剂盒测量DJ-1和HE4的血清浓度。通过曼-惠特尼检验评估EC患者和HC之间的差异，并通过斯皮尔曼相关性检验相关性。使用受试者工作特征（ROC）曲线分析评估诊断性能。

结果

发现EC患者的血清DJ-1浓度高于HC（9533.6对1988.5 pg/mL；P<.0001）。ROC曲线下面积（ROC-AUC）为0.95（P<.0001）。在3654 pg/mL的临界值时，敏感性和特异性分别为0.89和0.90。EC患者的HE4血清水平高于HC（75.3对56.2 pmol/L；P=.019），AUC为0.66（P=.020）。两种标志物联合获得的AUC为0.96（P<.0001）。