Biomolecular Measurement Division and Chemical Sciences Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
Statistical Engineering Division, National Institute of Standards and Technology , Gaithersburg, Maryland 20899, United States.
Anal Chem. 2017 May 2;89(9):4907-4913. doi: 10.1021/acs.analchem.6b05168. Epub 2017 Apr 13.
The National Institute of Standards and Technology (NIST) has developed Standard Reference Material (SRM) 972a Vitamin D Metabolites in Frozen Human Serum as a replacement for SRM 972, which is no longer available. SRM 972a was developed in collaboration with the National Institutes of Health's Office of Dietary Supplements. In contrast to the previous reference material, three of the four levels of SRM 972a are composed of unmodified human serum. This SRM has certified and reference values for the following 25-hydroxyvitamin D [25(OH)D] species: 25(OH)D, 25(OH)D, and 3-epi-25(OH)D. The value assignment and certification process included three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). The value assignment methods employed have been modified from those utilized for the previous SRM, and all three approaches now incorporate chromatographic resolution of the stereoisomers, 25(OH)D and 3-epi-25(OH)D.
美国国家标准与技术研究院 (NIST) 已开发出冷冻人血清中维生素 D 代谢物标准参考物质 (SRM)972a，以替代不再供应的 SRM972。SRM972a 是与美国国立卫生研究院膳食补充剂办公室合作开发的。与之前的参考物质相比，SRM972a 的四个水平中有三个是由未经修饰的人血清组成。该标准物质具有以下 25-羟基维生素 D [25(OH)D] 物质的认证和参考值：25(OH)D、25(OH)D2 和 3-表-25(OH)D。赋值和认证过程包括三种同位素稀释质谱分析方法，在 NIST 和疾病控制与预防中心 (CDC) 进行测量。所采用的赋值方法已经过修改，不再使用之前的 SRM 中使用的方法，现在所有三种方法都结合了 25(OH)D 和 3-表-25(OH)D 的立体异构体的色谱分离。
