Lee C Y, Few J D, James V H
Department of Chemical Pathology, St Mary's Hospital Medical School, London, England.
J Steroid Biochem. 1988 May;29(5):511-7.
A radioimmunoassay (RIA) for 18-hydroxycorticosterone (18OHB) is described using antibodies raised against 18OHB-3-CMO conjugated to bovine albumen and an iodine-125 labelled ligand. Extracts of plasma and saliva required thin-layer chromatographic purification prior to RIA. These assays have been validated in terms of precision, accuracy and specificity. The concentration of 18OHB in saliva is approx 20% of that in plasma and the two values are correlated. Thus, in a series of 20 healthy subjects the saliva and plasma concentration were 169 +/- 92 and 904 +/- 621 pmol.l-1 respectively with r = 0.72 (P less than 0.001). When the diurnal patterns of 18OHB, aldosterone and glucocorticoid (cortisol + cortisone) concentrations in saliva were investigated the patterns of 18OHB and aldosterone were usually similar though the 18OHB:aldosterone ratio can vary considerably. These and similar data suggest that the secretion of 18OHB and aldosterone are not completely synchronous. Our results show that saliva is a suitable body fluid in which to estimate 18OHB concentration and this measurement gives a useful reflection of the plasma 18OHB concentration. This technique should facilitate the investigation of the physiological role of this compound.
本文描述了一种针对18-羟皮质酮（18OHB）的放射免疫分析（RIA）方法，该方法使用针对与牛血清白蛋白偶联的18OHB-3-CMO产生的抗体以及碘-125标记的配体。血浆和唾液提取物在进行RIA之前需要进行薄层层析纯化。这些分析方法在精密度、准确性和特异性方面均已得到验证。唾液中18OHB的浓度约为血浆中的20%，且两者的值具有相关性。因此，在一组20名健康受试者中，唾液和血浆浓度分别为169±92和904±621 pmol·l-1，r = 0.72（P<0.001）。当研究唾液中18OHB、醛固酮和糖皮质激素（皮质醇+可的松）浓度的昼夜变化模式时，18OHB和醛固酮的模式通常相似，尽管18OHB:醛固酮的比值可能有很大差异。这些以及类似的数据表明，18OHB和醛固酮的分泌并非完全同步。我们的结果表明，唾液是一种适合用于估计18OHB浓度的体液，这种测量能够有效反映血浆18OHB浓度。该技术应有助于对该化合物的生理作用进行研究。
