基于JC-1染色的公猪和公牛精子冷冻保存过程中线粒体功能的比较
Comparison of Mitochondrial Function in Boar and Bull Spermatozoa Throughout Cryopreservation Based on JC-1 Staining.
作者信息
Hu C H, Zhuang X J, Wei Y M, Zhang M, Lu S S, Lu Y Q, Yang X G, Lu K H
机构信息
College of Animal Science and Technology, Guangxi University, Nanning, China.
出版信息
Cryo Letters. 2017 Jan/Feb;38(1):75-79.
BACKGROUND
Poor reproductivity hampers the commercialization of cryopreserved boar semen.
OBJECTIVE
This study was to determine the differences in the sperm mitochondrial function between boar and bull semen at different cryopreservation stages.
MATERIALS AND METHODS
Boar and bull fresh, equilibrated, and frozen-thawed spermatozoa were evaluated for mitochondrial function using JC-1 under a fluorescent microscope.
RESULTS
Bull and boar percentage of spermatozoa staining green (PSSG) showed no difference between fresh and equilibrated semen (P> 0.05). However, frozen-thawed bull and boar semen demonstrated significantly higher PSSG (P < 0.01) than fresh and equilibrated semen. Frozen-thawed boar semen represented a significantly higher PSSG (P < 0.01) than bull semen.
CONCLUSION
Negative cryopreservation influence on boar and bull spermatozoa was not significantly produced by pre-freezing procedures, but rather by freezing and thawing. Cryopreservation has more pronounced negative effects on boar than on bull spermatozoa, which partly explains lagged commercialization of frozen boar semen.
背景
繁殖力低下阻碍了冷冻公猪精液的商业化。
目的
本研究旨在确定公猪和公牛精液在不同冷冻保存阶段精子线粒体功能的差异。
材料与方法
使用JC-1在荧光显微镜下对公猪和公牛新鲜、平衡及冻融后的精子进行线粒体功能评估。
结果
公牛和公猪精子染成绿色的百分比（PSSG）在新鲜精液和平衡精液之间无差异（P>0.05）。然而，冻融后的公牛和公猪精液的PSSG显著高于新鲜和平衡精液（P<0.01）。冻融后的公猪精液的PSSG显著高于公牛精液（P<0.01）。
结论
冷冻保存对公猪和公牛精子的负面影响并非由冷冻前程序显著产生，而是由冷冻和解冻造成。冷冻保存对公猪精子的负面影响比对公牛精子更明显，这部分解释了冷冻公猪精液商业化滞后的原因。
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