Hamacher Tanja, Berendsen Johanna T W, Kruit Stella A, Broekhuijse Marleen L W J, Segerink Loes I
BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre, Faculty of Electrical Engineering, Mathematics and Computer Science, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Biomicrofluidics. 2020 Aug 3;14(4):044111. doi: 10.1063/5.0013919. eCollection 2020 Jul.
The use of microfluidics in artificial reproductive technologies for manipulation or assessment of spermatozoa is unique in the sense that it is not always an end point measurement and the sample may be used afterward. During microfluidic processing, spermatozoa are exposed to shear stress, which may harm viability and functioning of spermatozoa. The shear stresses during general microfluidic processing steps were calculated and compared to estimated shear stresses during ejaculation. The viability of boar and bull spermatozoa after microfluidic processing was studied and compared to the typical handling method (centrifugation) and to a control (the sample in a tube at the same temperature). The boar spermatozoa showed a small but significant decrease in viability of 6% after microfluidic handling. Bull spermatozoa proved to be less susceptible to shear stress and were not significantly affected by microfluidic processing. These data indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than the literature values reported for flow cytometry and comparable to the impact of centrifugation.
在人工生殖技术中，微流控技术用于精子的操作或评估具有独特之处，即它并不总是终点测量，且样本之后可能会被继续使用。在微流控处理过程中，精子会受到剪切应力，这可能会损害精子的活力和功能。计算了一般微流控处理步骤中的剪切应力，并将其与射精过程中的估计剪切应力进行比较。研究了公猪和公牛精子在微流控处理后的活力，并将其与典型处理方法（离心）及对照（相同温度下置于管中的样本）进行比较。公猪精子在微流控处理后活力出现了虽小但显著的6%的下降。公牛精子被证明对剪切应力不太敏感，微流控处理对其没有显著影响。这些数据表明，微流控处理对公猪和公牛精子活力的影响小于流式细胞术报道的文献值，且与离心的影响相当。
