Queern Stacy Lee, Aweda Tolulope Aramide, Massicano Adriana Vidal Fernandes, Clanton Nicholas Ashby, El Sayed Retta, Sader Jayden Andrew, Zyuzin Alexander, Lapi Suzanne Elizabeth
Department of Chemistry, Washington University in St. Louis, USA; Department of Radiology, University of Alabama at Birmingham, USA.
Department of Radiology, University of Alabama at Birmingham, USA.
Nucl Med Biol. 2017 Jul;50:11-16. doi: 10.1016/j.nucmedbio.2017.03.004. Epub 2017 Mar 23.
An increasing interest in zirconium-89 (Zr) can be attributed to the isotope's half-life which is compatible with antibody imaging using positron emission tomography (PET). The goal of this work was to develop an efficient means of production for Zr that provides this isotope with high radionuclidic purity and specific activity. We investigated the irradiation of yttrium sputtered niobium coins and compared the yields and separation efficiency to solid yttrium coins. The sputtered coins were irradiated with an incident beam energy of 17.5MeV or 17.8MeV providing a degraded transmitted energy through an aluminum degrader of 12.5MeV or 12.8MeV, respectively, with various currents to determine optimal cyclotron conditions for Zr production. Dissolution of the solid yttrium coin took 2h with 50mL of 2M HCl and dissolution of the sputtered coin took 15-30min with 4mL of 2M HCl. During the separation of Zr from the solid yttrium coins, 77.9 ± 11.2% of the activity was eluted off in an average of 7.3mL of 1M oxalic acid whereas for the sputtered coins, 91 ± 6% was eluted off in an average of 1.2mL of 1M oxalic acid with 100% radionuclidic purity. The effective specific activity determined via DFO-SCN titration from the sputtered coins was 108±7mCi/μmol as compared to 20.3mCi/μmol for the solid yttrium coin production. ICP-MS analysis of the yttrium coin and the sputtered coins showed 99.99% yttrium removed with 178μg of yttrium in the final solution and 99.93-100% of yttrium removed with remaining range of 0-42μg of yttrium in the final solution, respectively. The specific activity calculated for the solid coin and 3 different sputtered coins using the concentration of Zr found via ICP-MS was 140±2mCi/μmol, 300±30mCi/μmol, 410±60mCi/μmol and 1719±5mCi/μmol, respectively. Labeling yields of the Zr produced via sputtered targets for Zr- DFO-trastuzumab were >98%. Overall, these results show the irradiation of yttrium sputtered niobium coins is a highly effective means for the production of Zr.
对锆 - 89（Zr）的兴趣与日俱增，这归因于该同位素的半衰期，它与使用正电子发射断层扫描（PET）的抗体成像相兼容。这项工作的目标是开发一种高效的Zr生产方法，以提供具有高放射性核纯度和比活度的该同位素。我们研究了溅射钇铌硬币的辐照情况，并将产率和分离效率与固态钇硬币进行了比较。用能量为17.5MeV或17.8MeV的入射束辐照溅射硬币，通过铝衰减器后分别提供12.5MeV或12.8MeV的衰减透射能量，并改变不同电流以确定Zr生产的最佳回旋加速器条件。固态钇硬币用50mL 2M HCl溶解需2小时，而溅射硬币用4mL 2M HCl溶解需15 - 30分钟。在从固态钇硬币中分离Zr的过程中，平均7.3mL 1M草酸洗脱了77.9±11.2%的活度，而对于溅射硬币，平均1.2mL 1M草酸以100%的放射性核纯度洗脱了91±6%的活度。通过DFO - SCN滴定法测定，溅射硬币的有效比活度为108±7mCi/μmol，而固态钇硬币生产的比活度为20.3mCi/μmol。对钇硬币和溅射硬币的ICP - MS分析表明，最终溶液中分别去除了99.99%的钇，剩余178μg钇，以及去除了99.93 - 100%的钇，最终溶液中剩余钇的范围为0 - 42μg。使用通过ICP - MS测定的Zr浓度计算，固态硬币和3种不同溅射硬币的比活度分别为140±2mCi/μmol、300±30mCi/μmol、410±60mCi/μmol和1719±5mCi/μmol。通过溅射靶生产的Zr用于Zr - DFO - 曲妥珠单抗的标记产率>98%。总体而言，这些结果表明溅射钇铌硬币的辐照是生产Zr的一种高效方法。
