Tong Xiaoyu, Burton Inari S, Jurvelin Jukka S, Isaksson Hanna, Kröger Heikki
Kuopio Musculoskeletal Research Unit (KMRU), Institute of Clinical Medicine, University of Eastern Finland, POB 1627, FIN-70211 Kuopio, Finland; Department of Applied Physics, University of Eastern Finland, POB 1627, FIN-70211 Kuopio, Finland.
Department of Applied Physics, University of Eastern Finland, POB 1627, FIN-70211 Kuopio, Finland; Diagnostic Imaging Centre, Kuopio University Hospital, POB 100, FIN-70029 KYS, Kuopio, Finland.
Bone Rep. 2016 Nov 28;6:9-16. doi: 10.1016/j.bonr.2016.11.004. eCollection 2017 Jun.
The cortical characteristics of the iliac crest in male have rarely been investigated with quantitative histomorphometry. Also it is still unknown how cortical microarchitecture may vary between the iliac crest and fractures related sites at the proximal femur. We studied the microarchitecture of both external and internal cortices within the iliac crest, and compared the results with femoral neck and subtrochanteric femoral shaft sites.
Undecalcified histological sections of the iliac crest were obtained bicortically from cadavers ( = 20, aged 18-82 years, males). They were cut (7 μm) and stained using modified Masson-Goldner stain. Histomorphometric parameters of cortical bone were analysed with low (× 50) and high (× 100) magnification, after identifying cortical bone boundaries using our previously validated method. Within cortical bone area, only complete osteons with typical concentric lamellae and cement line were selected and measured.
At the iliac crest, the mean cortical width of external cortex was higher than at the internal cortex (p < 0.001). Also, osteon structural parameters, e.g. mean osteonal perimeter, were higher in the external cortex (p < 0.05). In both external and internal cortices, pore number per cortical bone area was higher in young subjects (≤ 50 years) (p < 0.05) while mean pore perimeter was higher in the old subjects (> 50 years) (p < 0.05). Several cortical parameters (e.g. osteon area per cortical bone area, pore number per cortical area) were the lowest in the femoral neck (p < 0.05). The maximal osteonal diameter and mean wall width were the highest in the external cortex of the iliac crest (p < 0.05), and the mean cortical width, osteon number per cortical area were the highest in the subtrochanteric femoral shaft (p < 0.05). Some osteonal structural parameters (e.g. min osteonal diameter) were significantly positively correlated (0.29 ≤ R ≤ 0.45, p < 0.05) between the external iliac crest and the femoral neck.
This study reveals heterogeneity in cortical microarchitecture between the external and internal iliac crest cortices, as well as between the iliac crest, the femoral neck and the subtrochanteric femoral shaft. Standard iliac crest biopsy does not reflect accurately cortical microarchitecture of other skeletal sites.
男性髂嵴骨皮质特征鲜少通过定量组织形态计量学进行研究。此外，髂嵴与股骨近端骨折相关部位的骨皮质微结构如何变化仍不清楚。我们研究了髂嵴内外皮质的微结构，并将结果与股骨颈和股骨转子下骨干部位进行比较。
从尸体（n = 20，年龄18 - 82岁，男性）双侧髂嵴获取未脱钙组织切片。切片厚度为7μm，采用改良的马森 - 戈德纳染色法进行染色。使用我们之前验证过的方法确定骨皮质边界后，在低倍（×50）和高倍（×100）放大倍数下分析骨皮质的组织形态计量学参数。在骨皮质区域内，仅选择并测量具有典型同心板层和黏合线的完整骨单位。
在髂嵴处，外侧皮质的平均皮质宽度高于内侧皮质（p < 0.001）。此外，外侧皮质的骨单位结构参数，如平均骨单位周长，更高（p < 0.05）。在外侧和内侧皮质中，年轻受试者（≤50岁）的每骨皮质面积孔隙数更高（p < 0.05），而老年受试者（>50岁）的平均孔隙周长更高（p < 0.05）。几个骨皮质参数（如每骨皮质面积的骨单位面积、每皮质面积的孔隙数）在股骨颈处最低（p < 0.05）。最大骨单位直径和平均壁宽在髂嵴外侧皮质中最高（p < 0.05），平均皮质宽度、每皮质面积的骨单位数量在股骨转子下骨干中最高（p < 0.05）。髂嵴外侧与股骨颈之间的一些骨单位结构参数（如最小骨单位直径）呈显著正相关（0.29≤R≤0.45，p < 0.05）。
本研究揭示了髂嵴内外侧皮质之间以及髂嵴、股骨颈和股骨转子下骨干之间骨皮质微结构的异质性。标准的髂嵴活检不能准确反映其他骨骼部位的骨皮质微结构。
