Rossie K M, Sheridan J F, Barthold S W, Tutschka P J
Department of Medicine, Ohio State University, Columbus 43210.
Transplantation. 1988 Jun;45(6):1012-6. doi: 10.1097/00007890-198806000-00003.
The effect of a localized viral infection on the occurrence of graft-vs.-host disease (GVHD) was examined in allogeneic rat bone marrow chimeras (ACI/LEW). Animals without clinical evidence of GVHD, 62 days after bone marrow transplant, were infected in salivary and lacrimal glands with sialodacryoadenitis virus (SDAV), and sacrificed 8-25 days postinfection. Using established histologic criteria, GVHD was found more frequently in salivary and lacrimal glands of SDAV-infected chimeras than uninfected chimeras. Skin and oral mucosa, tissues not infected by the virus, showed no differences in occurrence of GVHD, suggesting that the viral infection induced only local and not systemic GVHD. GVHD and SDAV infection, which are histologically similar, were differentiated by examining tissues for SDAV antigen using immunoperoxidase technique. Histologic changes were present for at least 1 week longer than viral antigen, suggesting they represented GVHD rather than viral infection. GVHD and SDAV infection were also differentiated by looking for a histologic feature characteristic of GVHD and not found in SDAV infection (periductal lymphocytic infiltrate). This was found in SDAV-infected chimeras more frequently than uninfected chimeras, suggesting that the viral infection somehow induced GVHD. Results showed a localized increase in the occurrence of GVHD subsequent to localized viral infection.
在异基因大鼠骨髓嵌合体（ACI/LEW）中研究了局部病毒感染对移植物抗宿主病（GVHD）发生的影响。骨髓移植62天后无GVHD临床证据的动物，在唾液腺和泪腺感染涎泪腺炎病毒（SDAV），并在感染后8至25天处死。根据既定的组织学标准，发现SDAV感染的嵌合体的唾液腺和泪腺中GVHD的发生频率高于未感染的嵌合体。皮肤和口腔黏膜，即未被病毒感染的组织，在GVHD的发生上没有差异，这表明病毒感染仅诱发局部而非全身性GVHD。通过使用免疫过氧化物酶技术检查组织中的SDAV抗原，区分了在组织学上相似的GVHD和SDAV感染。组织学变化比病毒抗原至少多存在1周，这表明它们代表GVHD而非病毒感染。还通过寻找GVHD特有的且在SDAV感染中未发现的组织学特征（导管周围淋巴细胞浸润）来区分GVHD和SDAV感染。在SDAV感染的嵌合体中比未感染的嵌合体更频繁地发现这种情况，这表明病毒感染以某种方式诱发了GVHD。结果显示局部病毒感染后GVHD的发生率局部增加。
