Shang Shuxin, Li Wei, Qin Xue, Zhang Shu, Liu Yinkun
Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China;; Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
J Cancer. 2017 Mar 7;8(5):887-893. doi: 10.7150/jca.17747. eCollection 2017.
Aberrant fucosylation plays a functional role in regulating ontogeny and celluar differentiation and are differentially regulated in cancerous condition, which could provide hallmarks for cancer diagnostics and surveillance. We previously developed a magnetic beads-based lectin ELISA system to measure fucosylated haptoglobin (Hp), which has been reported to be a cancer biomarker through a series of glycoproteomic analysis. In this study, serum fucosylated Hp ratios were measured using our ELISA kit in a separate cohort of 260 patients independently, including 130 healthy controls and 130 patients with hepatocellular carcinoma (HCC). Fucosylated Hp /Hp ratio (levels of fucosylated Hp /levels of protein Hp) and ELISA Index (OD value of fucosylated Hp /OD value of protein Hp) were calculated respectively to reflect Hp fucosylation level on its protein level. Our data showed that fucosylated Hp /Hp ratio (AUC=0.8449) and ELISA Index (AUC=0.8581) had better performance in distinguishing HCC from controls, which indicated that fucosylated Hp ratios could improve the diagnosis and prediction of HCC even with a low level of alpha-fetoprotein (AFP). Additionally, the combination analysis of AFP and fucosylated Hp ratios increased the AUC value for HCC diagnosis.
异常岩藻糖基化在调节个体发育和细胞分化中发挥功能性作用，并且在癌症状态下受到不同调控，这可为癌症诊断和监测提供标志。我们之前开发了一种基于磁珠的凝集素ELISA系统来测量岩藻糖化触珠蛋白（Hp），通过一系列糖蛋白质组学分析，它已被报道为一种癌症生物标志物。在本研究中，我们使用ELISA试剂盒在一个独立的包含260名患者的队列中测量血清岩藻糖化Hp比率，其中包括130名健康对照和130名肝细胞癌（HCC）患者。分别计算岩藻糖化Hp/Hp比率（岩藻糖化Hp水平/蛋白质Hp水平）和ELISA指数（岩藻糖化Hp的OD值/蛋白质Hp的OD值）以反映蛋白质水平上的Hp岩藻糖基化水平。我们的数据表明，岩藻糖化Hp/Hp比率（AUC = 0.8449）和ELISA指数（AUC = 0.8581）在区分HCC与对照方面具有更好的性能，这表明即使在甲胎蛋白（AFP）水平较低的情况下，岩藻糖化Hp比率也可以改善HCC的诊断和预测。此外，AFP与岩藻糖化Hp比率的联合分析增加了HCC诊断的AUC值。
