Faculty of Dentistry, The University of Hong Kong, Hong Kong.
Dr. D Y Patil Dental College and Hospital, Pune, India.
Oral Dis. 2017 Oct;23(7):926-940. doi: 10.1111/odi.12676. Epub 2017 May 24.
To profile salivary microbiomes of an urban-living, healthy Indian cohort and explore associations with proinflammatory status.
Fifty-one clinically healthy Indian subjects' salivary microbiomes were analyzed using 16S rRNA Illumina MiSeq sequencing. Community distribution was compared with salivary data from the Human Microbiome Project (HMP). Indian subjects were clustered using microbiome-based "partitioning along medoids" (PAM), and relationships of interleukin-1 beta levels with community composition were analyzed.
Indian subjects presented higher phylogenetic diversity than HMP. Several taxa associated with traditional societies gut microbiomes (Bacteroidales, Paraprevotellaceae, and Spirochaetaceae) were raised. Bifidobacteriaceae and Lactobacillaceae were approximately fourfold greater. A PAM cluster enriched in several Proteobacteria, Actinobacteria, and Bacilli taxa and having almost twofold higher Prevotella to Bacteroides ratio showed significant overrepresentation of subjects within the highest quartile of salivary interleukin-1 beta levels. Abiotrophia, Anaerobacillus, Micrococcus, Aggregatibacter, Halomonas, Propionivivrio, Paracoccus, Mannhemia, unclassified Bradyrhizobiaceae, and Caulobacteraceae were each significant indicators of presence in the highest interleukin-1 beta quartile. 2 OTUs representing Lactobacillus fermentum and Cardiobacterium hominis significantly correlated with interleukin-1 beta levels.
The salivary microbiome of this urban-dwelling Indian cohort differed significantly from that of a well-studied Western cohort. Specific community patterns were putatively associated with subclinical inflammation levels.
分析城市居住的健康印度人群的唾液微生物组，并探索其与促炎状态的关联。
使用 16S rRNA Illumina MiSeq 测序分析 51 名临床健康印度受试者的唾液微生物组。将群落分布与人类微生物组计划（HMP）中的唾液数据进行比较。使用基于微生物组的“中位数划分”（PAM）对印度受试者进行聚类，并分析白细胞介素-1β水平与群落组成的关系。
印度受试者的系统发育多样性高于 HMP。一些与传统社会肠道微生物组相关的分类群（拟杆菌目、副拟杆菌科和螺旋体科）被提出。双歧杆菌科和乳杆菌科约增加了四倍。一个富含几种变形菌门、放线菌门和芽孢杆菌门的 PAM 聚类，且普雷沃氏菌与拟杆菌的比例几乎高出两倍，其唾液白细胞介素-1β水平最高四分位数的受试者数量明显增加。生物素缺陷菌、厌氧杆菌、微球菌、聚集杆菌、盐单胞菌、丙酸杆菌、副球菌、曼海姆菌、未分类的慢生根瘤菌科和钙杆菌科是白细胞介素-1β水平最高四分位数存在的显著指标。代表发酵乳杆菌和人心杆菌的 2 个 OTU 与白细胞介素-1β水平显著相关。
该城市居住的印度人群的唾液微生物组与经过充分研究的西方人群有显著差异。特定的群落模式与亚临床炎症水平存在推测性关联。
