1 Department Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Santiago de Compostela (USC) , Santiago de Compostela, Spain .
2 Department of Pharmacy, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS) , Santiago de Compostela, Spain .
J Ocul Pharmacol Ther. 2017 Apr;33(3):202-209. doi: 10.1089/jop.2016.0053.
Acanthamoeba keratitis causes frequent epithelial lesions that fully expose the corneal stroma. The aim of this study was to determine the toxic profile of chlorhexidine and propamidine eye drops.
We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. Additional studies such as a classic cell viability test (WST-1), a bovine corneal opacity and permeability assay, and an irritation eye study (Hen's Egg Test [HET]) have been made.
Both eye drop formulations showed a time- and concentration-dependent toxicity profile, in which long periods and high concentrations were more detrimental to cells. In prolonged times of exposure, propamidine is more harmful to cells than chlorhexidine. On the contrary, no irritation has been detected in using the HET-chorioallantoic membrane test and no alterations in the corneal transparency nor permeability was produced by the treatment with both eye drops.
In culture assay, chlorhexidine eye drops have proven to be less cytotoxic than Brolene for a long contact period of time, but no signs of irritation or alterations in transparency or permeability have been observed in the cornea after both treatments.
棘阿米巴角膜炎常导致角膜上皮完全暴露于基质层的病变。本研究旨在确定洗必泰与丙脒定滴眼液的毒性特征。
我们在细胞培养中使用原代人角膜基质细胞，并结合一种通过阻抗分析评估动态实时细胞毒性的新技术。还进行了其他研究，如经典细胞活力测试（WST-1）、牛眼角膜混浊和通透性测定以及眼部刺激性研究（鸡胚绒毛尿囊膜试验[HET]）。
两种滴眼液均呈现出时间和浓度依赖性的毒性特征，其中长时间和高浓度对细胞的危害性更大。在长时间暴露的情况下，丙脒定对细胞的危害性大于洗必泰。相反，HET-鸡胚绒毛尿囊膜试验未检测到刺激性，两种滴眼液处理后角膜的透明度和通透性均未发生改变。
在培养物检测中，与 Brolene 相比，洗必泰滴眼液在长时间接触时的细胞毒性更小，但两种处理后角膜均未出现刺激迹象或透明度或通透性改变。
