Department of Chemical Engineering, Northeastern University, 360 Huntington Ave., Boston, MA 02151, USA.
Department of Medicine, University of California San Francisco, 405 Parnassus Ave., San Francisco, CA 94143, USA.
J Colloid Interface Sci. 2017 Aug 1;499:189-201. doi: 10.1016/j.jcis.2017.03.109. Epub 2017 Mar 30.
Bile micelles are thought to mediate intestinal absorption, in part by providing a phase into which compounds can partition. Solubilizing capacity of bile micelles is enhanced during the digestion of fat rich food. We hypothesized that the intestinal digestion of triglycerides causes an increase in volume of micelles that can be quantitatively monitored over the course of digestion using small-angle neutron scattering (SANS), and that SANS can enable evaluation of the contribution of each of the components present during digestion to the size of micelles.
SANS was used to characterize the size and shape of micelles present prior to and during the in vitro simulated intestinal digestion of a model food-associated lipid, triolein.
Pre-lipolysis mixtures of a bile salt and phospholipid simulating bile concentrations in fed conditions were organized in micelles with an average volume of 40 nm. During lipolysis, the micelle volume increased 2.5-fold over a 2-h digestion period due to growth in one direction as a result of insertion of monoglycerides and fatty acids. These efforts represent a basis for quantitative mechanistic understanding of changes in solubilizing capacity of the intestinal milieu upon ingestion of a fat-rich meal.
胆微团被认为通过提供化合物可以分配的相部分介导肠吸收。富含脂肪的食物消化期间胆微团的增溶能力增强。我们假设，甘油三酯的肠消化导致微团体积增加，可以使用小角中子散射（SANS）在消化过程中定量监测，并且 SANS 可以评估在消化过程中存在的每个成分对微团大小的贡献。
SANS 用于表征在体外模拟肠消化模型食物相关脂质三油酸甘油酯之前和期间存在的微团的大小和形状。
在进食条件下模拟胆汁浓度的胆汁盐和磷脂的预脂解混合物以平均体积为 40nm 的微团形式存在。在脂解过程中，由于单甘油脂和脂肪酸的插入导致一个方向上的生长，微团体积在 2 小时的消化过程中增加了 2.5 倍。这些努力为定量理解摄入高脂肪餐后肠环境增溶能力的变化提供了基础。
