Sardar Ahmed, Basireddy Sreekanth Reddy, Navaz Anwar, Singh Manisha, Kabra Vasanti
Assistant Professor, Department of Microbiology, SVS Medical College , Mahbubnagar, Telangana, India .
Assistant Professor, Department of Microbiology, Kurnool Medical College , Kurnool, Andhra Pradesh, India .
J Clin Diagn Res. 2017 Feb;11(2):DC26-DC29. doi: 10.7860/JCDR/2017/23644.9440. Epub 2017 Feb 1.
Fosfomycin is a broad spectrum anti-microbial agent with activity against various bacterial agents. It is a bactericidal antibiotic which interferes with cell wall synthesis. Fosfomycin is rapidly absorbed orally and majority of the drug is excreted unchanged in urine with very high concentration levels achieved in urine after a single oral dose. Urine levels remain high for prolonged period which makes it a suitable drug in the treatment of Urinary Tract Infections (UTI). is the most common organism causing the UTI. With the inappropriate and inadvertent use of higher antibiotics, these bacterial isolates have acquired multidrug resistance for which treatment options are limited.
To evaluate the in-vitro activity of fosfomycin against uropathogenic and to compare its activity with the other anti-microbial agents.
This study was a prospective study done in the Department of Microbiology SVS Medical College, Mahbubnagar from Jan 2016 to Mar 2016. A total of 564 urine samples from suspected UTI cases were processed during the study period out of which 170 were isolated. Identification of the organisms were done by routine biochemical testing and antibiotic sensitivity testing was done by Kirby-Bauer disc diffusion testing according to the CLSI guidelines (M100-S23).
A total of 170 isolates were tested for antibiotic susceptibility. Out of 170 isolates 60 (35.30%) were isolated from males and 110 (64.70%) from females with male to female ratio of 1:1.83. Majority of the isolates were obtained from the age group of 21-30 years (25.8%). Antibiotics like fosfomycin, imipenem and methenamine mandelate showed the highest sensitivity with all the isolates (100%) being susceptible to these drugs. Whereas, least sensitivity was observed for amoxyclav (15.2%) followed by cefixime (16.4%) and norfloxacin (21%). Fosfomycin has shown very good in-vitro activity against all the tested isolates when compared with many other antibiotics.
In view of emergence of multidrug resistant pathogens, testing for old and forgotten antibiotics like fosfomycin is gaining importance. Because of its unique mechanism of action and low incidence of resistance it can be a potential therapeutic alternative in the treatment of UTI.
磷霉素是一种广谱抗菌剂，对多种细菌具有活性。它是一种干扰细胞壁合成的杀菌抗生素。磷霉素口服后吸收迅速，单次口服给药后，大部分药物以原形经尿液排出，尿液中可达到很高的浓度水平。尿液中的药物浓度在较长时间内保持较高，这使其成为治疗尿路感染（UTI）的合适药物。大肠杆菌是引起尿路感染最常见的病原体。随着高等级抗生素的不当和随意使用，这些细菌分离株已获得多重耐药性，治疗选择有限。
评估磷霉素对尿路致病性大肠杆菌的体外活性，并将其活性与其他抗菌剂进行比较。
本研究是2016年1月至2016年3月在马赫布卜纳加尔SVS医学院微生物学系进行的一项前瞻性研究。在研究期间，共处理了564份疑似尿路感染病例的尿液样本，其中分离出170株大肠杆菌。通过常规生化检测对分离出的细菌进行鉴定，并根据CLSI指南（M100-S23）采用 Kirby-Bauer 纸片扩散法进行药敏试验。
共对170株大肠杆菌分离株进行了药敏试验。在170株分离株中，60株（35.30%）分离自男性，110株（64.70%）分离自女性，男女比例为1:1.83。大多数分离株来自21至30岁年龄组（25.8%）。磷霉素、亚胺培南和孟德立胺等抗生素显示出最高的敏感性，所有分离株（100%）对这些药物敏感。而阿莫西林克拉维酸（15.2%）、头孢克肟（16.4%）和诺氟沙星（21%）的敏感性最低。与许多其他抗生素相比，磷霉素对所有测试分离株均显示出非常好的体外活性。
鉴于多重耐药病原体的出现，对像磷霉素这样古老且被遗忘的抗生素进行检测变得越来越重要。由于其独特的作用机制和低耐药发生率，它可能成为治疗尿路感染的一种潜在治疗选择。
