Center for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium(2); Laboratory of Industrial Water and Eco-Technology (LIWET), Faculty of Bioscience Engineering, Ghent University, Graaf Karel de Goedelaan 5, B-8500 Kortrijk, Belgium(3); ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas (CENAIM), Campus Gustavo Galindo, Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador(4); ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida, Campus Gustavo Galindo, Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador(5).
Laboratory of Industrial Water and Eco-Technology (LIWET), Faculty of Bioscience Engineering, Ghent University, Graaf Karel de Goedelaan 5, B-8500 Kortrijk, Belgium(3); Centre for the Study of Education, Technologies and Health (CSETH), Polytechnic Institute of Viseu, Av. J. M. Vale de Andrade, 3504-510 Viseu, Portugal(6).
N Biotechnol. 2017 Oct 25;39(Pt A):1-10. doi: 10.1016/j.nbt.2017.03.004. Epub 2017 Apr 4.
Treatment of upflow anaerobic sludge blanket (UASB) effluent from a paper mill in aerated activated sludge reactors involves high aeration costs. Moreover, this calcium-rich effluent leads to problematic scale formation. Therefore, a novel strategy for the aerobic treatment of paper mill UASB effluent in microalgal bacterial floc sequencing batch reactors (MaB-floc SBRs) is proposed, in which oxygen is provided via photosynthesis, and calcium is removed via bio-mineralization. Based on the results of batch experiments in the course of this study, a MaB-floc SBR was operated at an initial neutral pH. This SBR removed 58±21% organic carbon, 27±8% inorganic carbon, 77±5% nitrogen, 73±2% phosphorus, and 27±11% calcium. MaB-flocs contained 10±3% calcium, including biologically-influenced calcite crystals. The removal of calcium and inorganic carbon by MaB-flocs significantly decreased when inhibiting extracellular carbonic anhydrase (CA), an enzyme that catalyses the hydration and dehydration of CO. This study demonstrates the potential of MaB-floc SBRs for the alternative treatment of calcium-rich paper mill effluent, and highlights the importance of extracellular CA in this treatment process.
在曝气活性污泥反应器中处理来自造纸厂的上流式厌氧污泥床（UASB）废水会涉及到高昂的曝气成本。此外，这种富含钙的废水会导致严重的结垢问题。因此，本研究提出了一种在微藻-细菌絮体序批式反应器（MaB-floc SBR）中好氧处理造纸厂 UASB 废水的新策略，该策略通过光合作用提供氧气，并通过生物矿化去除钙。基于本研究中分批实验的结果，在初始中性 pH 条件下运行 MaB-floc SBR。该 SBR 去除了 58±21%的有机碳、27±8%的无机碳、77±5%的氮、73±2%的磷和 27±11%的钙。MaB-flocs 含有 10±3%的钙，包括受生物影响的方解石晶体。当抑制细胞外碳酸酐酶（CA）时，MaB-flocs 对钙和无机碳的去除显著下降，CA 是一种催化 CO 水合和脱水的酶。本研究证明了 MaB-floc SBR 作为替代处理富含钙的造纸厂废水的潜力，并强调了细胞外 CA 在该处理过程中的重要性。
