Suwalsky Mario, Duguet José, Speisky Hernán
Faculty of Chemical Sciences, University of Concepción, Concepción, Chile.
Centre of Biotechnology and Bioengineering, University of Chile, Santiago, Chile.
J Membr Biol. 2017 Jun;250(3):239-248. doi: 10.1007/s00232-017-9955-0. Epub 2017 Apr 6.
The antioxidant and antihemolytic properties contained in the leaves of Buddleja globosa (B. globosa), also known as "Matico," were determined. Aqueous extracts of leaves were assayed in human erythrocytes and molecular models of its membrane. The latter were bilayers built-up of lipids located in the outer and inner leaflets of the erythrocyte membrane. Observations by scanning electron microscopy showed that the extract altered the morphology of erythrocytes inducing the formation of crenated echinocytes. This result implied that the extract components were inserted into the outer leaflet of the cell membrane. This conclusion was confirmed by experiments carried out by fluorescence spectroscopy of red cell membranes and vesicles (LUV) of dimyristoylphosphatidylcholine (DMPC) and by X-ray diffraction of DMPC and dimyristoylphosphatidylethanolamine bilayers. Human erythrocytes were in vitro exposed to HClO, which is a natural powerful oxidant. Results demonstrated that low concentrations of B. globosa aqueous extract neutralized the harmful capacity of HClO. Hemolysis experiments also showed that the extract in very low concentrations reduced hemolysis induced by HClO.
测定了密花醉鱼草（Buddleja globosa）叶中含有的抗氧化和抗溶血特性，密花醉鱼草也被称为“马蒂科”。叶的水提取物在人红细胞及其膜的分子模型中进行了测定。后者是由位于红细胞膜外层和内层小叶中的脂质构成的双层膜。扫描电子显微镜观察表明，提取物改变了红细胞的形态，诱导了皱缩棘状红细胞的形成。这一结果表明提取物成分插入到了细胞膜的外层小叶中。通过红细胞膜和二肉豆蔻酰磷脂酰胆碱（DMPC）囊泡（大单层囊泡）的荧光光谱实验以及DMPC和二肉豆蔻酰磷脂酰乙醇胺双层膜的X射线衍射实验证实了这一结论。人红细胞在体外暴露于次氯酸（HClO），次氯酸是一种天然的强氧化剂。结果表明，低浓度的密花醉鱼草水提取物中和了次氯酸的有害能力。溶血实验还表明，极低浓度的提取物减少了由次氯酸诱导的溶血。
