Hutchison F N, Kaysen G A
Department of Medicine, Veteran's Administration Medical Center, Martinez, California.
Kidney Int. 1988 Apr;33(4):787-91. doi: 10.1038/ki.1988.68.
To determine if changes in dietary protein intake alter renal excretion of small molecular weight proteins in passive Heymann nephritis, 21 rats with passive Heymann nephritis were fed 8.5% protein for 12 days after injection with antiserum. Dietary protein intake was then increased to 40% in 10 rats (LP-HP) while 11 rats remained on 8.5% protein (LP-LP). Lysozymuria (UlysV) increased from 66.5 +/- 31.0 mcg/day to 457.5 +/- 98.0 mcg/day (P less than 0.001) after five days in LP-HP, but was unchanged in LP-LP. Albuminuria (UalbV) increased only in LP-HP, from 168 +/- 23 mg/day to 447 +/- 45 mg/day (P less than 0.001). Urinary lysozyme excretion correlated with UalbV (r = 0.737, P less than 0.001), and changes in UlysV correlated with changes in UalbV (r = 0.657, P less than 0.01). To determine whether the increase in UlysV was the direct effect of the change in diet, enalapril 40 mg/kg/day was administered to prevent the increase in UalbV that occurs when these rats are fed a high protein diet. Twelve rats were fed 8.5% (LP) and 10 were fed 40% protein (HP) from the time of injection with antiserum. Six LP (LPE) and five HP (HPE) received enalapril. UlysV was 873 +/- 391 mcg/day in HP and nearly undetectable in the other three groups. UalbV was significantly greater in HP (368 +/- 60 mg/day) compared to the other three groups (114 +/- 16 in LP, 136 +/- 44 in HPE, 95 +/- 21 in LPE.(ABSTRACT TRUNCATED AT 250 WORDS)
为了确定饮食蛋白质摄入量的变化是否会改变被动型海曼肾炎中小分子蛋白质的肾脏排泄情况，21只患有被动型海曼肾炎的大鼠在注射抗血清后，接受了12天8.5%蛋白质的饮食喂养。然后，10只大鼠的饮食蛋白质摄入量增加到40%（LP-HP组），而11只大鼠继续保持8.5%的蛋白质摄入量（LP-LP组）。LP-HP组中，五天后溶菌酶尿（UlysV）从66.5±31.0微克/天增加到457.5±98.0微克/天（P<0.001），而LP-LP组则无变化。蛋白尿（UalbV）仅在LP-HP组中增加，从168±23毫克/天增加到447±45毫克/天（P<0.001）。尿溶菌酶排泄与UalbV相关（r=0.737，P<0.001），UlysV的变化与UalbV的变化相关（r=0.657，P<0.01）。为了确定UlysV的增加是否是饮食变化的直接影响，给予40毫克/千克/天的依那普利以防止这些大鼠喂食高蛋白饮食时出现的UalbV增加。从注射抗血清时起，12只大鼠喂食8.5%蛋白质（LP组），10只大鼠喂食40%蛋白质（HP组）。6只LP组大鼠（LPE组）和5只HP组大鼠（HPE组）接受依那普利治疗。HP组的UlysV为873±391微克/天，其他三组几乎检测不到。与其他三组（LP组为114±16，HPE组为136±44，LPE组为95±21）相比，HP组的UalbV显著更高（368±60毫克/天）。（摘要截取自250字）
