Li Shuting, Shi Min, Zhao Jingjin, Zhang Liangliang, Huang Yong, Zhao Shulin
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin, P. R. China.
Electrophoresis. 2017 Jul;38(13-14):1780-1787. doi: 10.1002/elps.201600396. Epub 2017 May 15.
An enzyme and antibody dual labeled gold nanoparticles enhancing chemiluminescence strategy was developed for highly sensitive CE immunoassay (IA) of prostate-specific antigen (PSA). In this work, gold nanoparticles were labeled with horseradish peroxidase and antiprostate specific antigen-antibody, and used as the marker (Ab ). After PSA (antigen, Ag) was added into the system, a noncompetitive immune reaction was happen between Ab and Ag to form an immune complex (Ag-Ab ). Subsequently, the obtained Ag-Ab and unreacted Ab were separated by CE, and the chemiluminescence intensity of Ag-Ab was used to estimate PSA concentration. The calibration curve showed a good linearity in the range of 0.25-10 ng/mL. Based on a S/N of 3, the detection limit for PAS was estimated to be 0.092 ng/mL. Proposed CE method was applied for PSA quantification in human serum samples from healthy volunteers and patients with prostate cancer. The obtained results demonstrated that the proposed CE method may serve as an alternative tool for clinical analysis of PSA.
开发了一种酶和抗体双标记金纳米颗粒增强化学发光策略，用于前列腺特异性抗原（PSA）的高灵敏度毛细管电泳免疫分析（IA）。在这项工作中，用辣根过氧化物酶和抗前列腺特异性抗原抗体标记金纳米颗粒，并用作标记物（Ab*）。将PSA（抗原，Ag）加入系统后，Ab与Ag之间发生非竞争性免疫反应，形成免疫复合物（Ag-Ab）。随后，通过毛细管电泳分离得到的Ag-Ab和未反应的Ab，并利用Ag-Ab*的化学发光强度来估算PSA浓度。校准曲线在0.25-10 ng/mL范围内呈现良好的线性。基于3的信噪比，PAS的检测限估计为0.092 ng/mL。所提出的毛细管电泳方法应用于健康志愿者和前列腺癌患者的人血清样本中PSA的定量分析。所得结果表明，所提出的毛细管电泳方法可作为PSA临床分析的替代工具。
