Silva R C, Liebel S, de Oliveira H H P, Ramsdorf W A, Garcia J R E, Azevedo S M F O, Magalhães V F, Oliveira Ribeiro C A, Filipak Neto F
Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil.
Departamento de Biologia Estrutural, Molecular e Genética. Bloco M. Setor de Ciências Biológicas e da Saúde, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil, CEP 84030-900.
Fish Physiol Biochem. 2017 Oct;43(5):1237-1244. doi: 10.1007/s10695-017-0367-y. Epub 2017 Apr 7.
Cylindrospermopsin (CYN) is a cyanotoxin that is cytotoxic to a wide variety of cells, particularly to the hepatocytes. In this study, the toxic effects of purified CYN were investigated in primary cultured hepatocytes of Neotropical fish Hoplias malabaricus. After isolation, attachment, and recovery for 72 h, the cells were exposed for 72 h to 0, 0.1, 1.0, 10, and 100 μg l of CYN. Then, cell viability and a set of oxidative stress biomarker responses were determined. Catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione S-transferase activities were not affected by exposure to CYN. Concentration-dependent decrease of glutathione reductase activity occurred for most CYN-exposed groups, whereas non-protein thiol content increased only for the highest CYN concentration. Lipid peroxidation, protein carbonylation, and DNA damage levels were not altered, but reactive oxygen species levels increased in the cells exposed to the highest concentration of CYN. Cell viability decreased in all the groups exposed to CYN. Thus, CYN may cause a slight change in redox balance, but it is not the main cause of cell death in H. malabaricus hepatocytes.
柱孢藻毒素（CYN）是一种对多种细胞具有细胞毒性的蓝藻毒素，尤其对肝细胞毒性较大。在本研究中，我们研究了纯化的CYN对新热带鱼马拉巴丽鱼原代培养肝细胞的毒性作用。细胞分离、贴壁并恢复72小时后，将细胞暴露于0、0.1、1.0、10和100μg/l的CYN中72小时。然后，测定细胞活力和一系列氧化应激生物标志物反应。过氧化氢酶、超氧化物歧化酶、葡萄糖-6-磷酸脱氢酶和谷胱甘肽S-转移酶的活性不受CYN暴露的影响。大多数暴露于CYN的组中，谷胱甘肽还原酶活性呈浓度依赖性下降，而仅在最高CYN浓度下非蛋白巯基含量增加。脂质过氧化、蛋白质羰基化和DNA损伤水平未改变，但暴露于最高浓度CYN的细胞中活性氧水平增加。所有暴露于CYN的组细胞活力均下降。因此，CYN可能会导致氧化还原平衡略有变化，但它不是马拉巴丽鱼肝细胞死亡的主要原因。
