Aizawa Shin-Ichi
Department of Life Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima, 727-0023, Japan.
Methods Mol Biol. 2017;1593:87-96. doi: 10.1007/978-1-4939-6927-2_6.
The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I describe protocols for the purification of the filament and hook-basal body from Salmonella enterica serovar Typhimurium.
细菌鞭毛是一种运动细胞器。鞭毛由三个主要结构组成：作为嵌入细胞膜和细胞壁的旋转引擎的基体、充当螺旋桨的长外部细丝以及作为连接它们的万向节的钩。我描述了从鼠伤寒沙门氏菌中纯化细丝和钩基体的方案。
