Inoue Yuichi
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai, Miyagi, 980-8577, Japan.
Sigmakoki Co., Ltd., Tokyo Head Office, 1-19-9 Midori, Sumidaku, Tokyo, 130-0021, Japan.
Methods Mol Biol. 2017;1593:163-174. doi: 10.1007/978-1-4939-6927-2_12.
Direct observation of the rotation of tethered cells using optical microscopy is a simple method to examine dynamics of the bacterial flagellar motor. The rotational speed indicates not only the existence of the rotary motor, but also approximate number of stators that are torque-generating units in a motor. Since "run" and "tumble" of the peritrichous cells as Escherichia coli are regulated by the counterclockwise rotation and the clockwise rotation, respectively, rotational direction of the tethered cell is an important clue to understand the chemotactic system of the cells.
使用光学显微镜直接观察系留细胞的旋转是一种检测细菌鞭毛马达动力学的简单方法。旋转速度不仅表明旋转马达的存在，还能大致显示马达中作为扭矩产生单元的定子数量。由于像大肠杆菌这样的周质鞭毛菌的“游动”和“翻滚”分别由逆时针旋转和顺时针旋转调节，系留细胞的旋转方向是理解细胞趋化系统的重要线索。
