嗜碱芽孢杆菌和类芽孢杆菌鞭毛马达的离子选择性

Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species.

作者信息

Takahashi Yuka, Ito Masahiro

机构信息

Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan.

Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Oura-gun, Gunma, 374-0193, Japan.

出版信息

Methods Mol Biol. 2017;1593:297-303. doi: 10.1007/978-1-4939-6927-2_24.

DOI:10.1007/978-1-4939-6927-2_24

PMID:28389964

Abstract

Many bacteria can swim using their flagella, which are filamentous organelles that extend from the cell surface. The flagellar motor is energized by either a proton (H) or sodium ion (Na) as the motive force. MotAB-type stators use protons, whereas MotPS- and PomAB-type stators use Na as the coupling ions. Recently, alkaliphilic Bacillus alcalophilus was shown to use potassium ions (K) and rubidium ions (Rb) for flagellar rotation, and the flagellar motor from Paenibacillus sp. TCA-20 uses divalent cations such as magnesium ions (Mg), calcium ions (Ca), and strontium ions (Sr) for coupling. In this chapter, we focus on how to identify the coupling ions for flagellar rotation of alkaliphilic Bacillus and Paenibacillus species.

摘要

许多细菌能够利用其鞭毛游动，鞭毛是从细胞表面伸出的丝状细胞器。鞭毛马达以质子（H⁺）或钠离子（Na⁺）作为驱动力来提供能量。MotAB型定子利用质子，而MotPS型和PomAB型定子则利用Na⁺作为耦合离子。最近研究表明，嗜碱嗜碱芽孢杆菌利用钾离子（K⁺）和铷离子（Rb⁺）进行鞭毛旋转，而类芽孢杆菌属TCA - 20的鞭毛马达则利用镁离子（Mg²⁺）、钙离子（Ca²⁺）和锶离子（Sr²⁺）等二价阳离子进行耦合。在本章中，我们重点关注如何鉴定嗜碱芽孢杆菌和类芽孢杆菌属物种鞭毛旋转的耦合离子。