Department of Anesthesia and Critical Care, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Department of Preventive Medicine, School of Environmental Science and Management, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
J Diabetes Investig. 2018 Jan;9(1):83-90. doi: 10.1111/jdi.12674. Epub 2017 May 18.
AIMS/INTRODUCTION: In previous studies, hydrogen gas (H administration has clearly shown effectiveness in inhibiting diabetes. Here, we evaluated whether subcutaneous injection of H shows enhanced efficacy against type 2 diabetes mellitus induced in mice by a high-fat diet and low-dose streptozotocin treatment.
H was injected subcutaneously at a dose of 1 mL/mouse/week for 4 weeks. Type 2 diabetes mellitus-associated parameters were then evaluated to determine the effectiveness of subcutaneous H administration.
The bodyweight of H -treated mice did not change over the course of the experiment. Compared with the untreated control animals, glucose, insulin, low-density lipoprotein and triglyceride levels in the serum were significantly lower in treated mice, whereas high-density lipoprotein cholesterol in the serum was significantly higher. Glucose tolerance and insulin sensitivity were both improved in H -treated mice. Diabetic nephropathy analysis showed significant reductions in urine volume, urinary total protein and β2-microglobulin, kidney/bodyweight ratio, and kidney fibrosis associated with subcutaneous injection of H .
Subcutaneous injection of H significantly improves type 2 diabetes mellitus and diabetic nephropathy-related outcomes in a mouse model, supporting further consideration of subcutaneous injection as a novel and effective route of clinical H administration.
目的/引言：在之前的研究中，氢气（H2）的给药已明确显示出抑制糖尿病的疗效。在这里，我们评估了每周 1 毫升/小鼠/次的皮下注射 H2 是否对高脂肪饮食和低剂量链脲佐菌素处理诱导的小鼠 2 型糖尿病具有增强的疗效。
H2 以 1 毫升/小鼠/周的剂量皮下注射 4 周，然后评估与 2 型糖尿病相关的参数，以确定皮下 H2 给药的效果。
H2 处理组小鼠的体重在实验过程中没有变化。与未处理的对照组动物相比，治疗组小鼠血清中的葡萄糖、胰岛素、低密度脂蛋白和甘油三酯水平显著降低，而高密度脂蛋白胆固醇显著升高。H2 处理组小鼠的葡萄糖耐量和胰岛素敏感性均得到改善。糖尿病肾病分析显示，H2 皮下注射后显著减少了尿量、尿总蛋白和β2-微球蛋白、肾脏/体重比以及肾脏纤维化。
皮下注射 H2 可显著改善小鼠 2 型糖尿病和糖尿病肾病相关的结局，支持进一步考虑皮下注射作为临床 H2 给药的一种新的有效途径。
