Zhang Meng, Chen Ying, Nie Lin, Jin Xiaofei, Fu Chunhua, Yu Longjiang
Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan 430074, PR China; Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan 430074, PR China.
Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan 430074, PR China; Key Laboratory of Molecular Biophysics Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Wuhan 430074, PR China.
Gene. 2017 Jul 15;620:66-74. doi: 10.1016/j.gene.2017.04.005. Epub 2017 Apr 6.
Taxus spp. are ancient gymnosperms that produce a unique secondary metabolite, namely, taxol, an anticancer drug. JAZ proteins are key regulators of the JA signaling pathway, which control taxol biosynthesis. However, the JAZ proteins of Taxus spp. are poorly studied. In this work, nine JAZ genes from Taxus chinensis were identified using our previous transcriptome data and named as TcJAZ1-TcJAZ9. Of these nine TcJAZ proteins, eight contain Jas and TIFY domains, and the Jas domain of TcJAZ6 is incomplete. Most TcJAZs and PsJAZs are not related to AtJAZs and OsJAZs. Phylogenetic analysis divided all JAZ proteins from Arabidopsis thaliana, Oryza sativa, Picea sitchensis, and T. chinensis into eight subgroups; gymnosperms JAZs were classified into subgroups V-VIII, and angiosperm JAZs were categorized into subgroups I-V. Three motifs of subgroups VI-VIII were identified in gymnosperm JAZs, indicating that gymnosperm JAZ proteins exhibit a different evolutionary process from those of angiosperms. The expression patterns of nine TcJAZs showed that TcJAZ2/3/8 was a key regulator, indicating their important roles in T. chinensis. Results revealed that gymnosperm JAZs differ from angiosperm JAZs in terms of molecular structure. Three novel conserved motifs were found in TcJAZs and PsJAZs. This study provides a basis for research on JA regulatory system in Taxus spp. and for elucidating the significance of JA signaling pathway to land plants.
红豆杉属植物是古老的裸子植物，能产生一种独特的次生代谢产物——抗癌药物紫杉醇。JAZ蛋白是茉莉酸（JA）信号通路的关键调节因子，控制着紫杉醇的生物合成。然而，对红豆杉属植物的JAZ蛋白研究较少。在这项研究中，利用我们之前的转录组数据从中国红豆杉中鉴定出9个JAZ基因，命名为TcJAZ1 - TcJAZ9。在这9个TcJAZ蛋白中，8个含有Jas和TIFY结构域，且TcJAZ6的Jas结构域不完整。大多数TcJAZ蛋白和云杉JAZ蛋白与拟南芥JAZ蛋白和水稻JAZ蛋白无关。系统发育分析将来自拟南芥、水稻、北美云杉和中国红豆杉的所有JAZ蛋白分为8个亚组；裸子植物JAZ蛋白被归类到亚组V - VIII，被子植物JAZ蛋白被归类到亚组I - V。在裸子植物JAZ蛋白中鉴定出亚组VI - VIII的三个基序，表明裸子植物JAZ蛋白呈现出与被子植物不同的进化过程。9个TcJAZ基因的表达模式表明TcJAZ2/3/8是关键调节因子，说明它们在中国红豆杉中发挥着重要作用。结果表明，裸子植物JAZ蛋白在分子结构上与被子植物JAZ蛋白不同。在TcJAZ蛋白和云杉JAZ蛋白中发现了三个新的保守基序。本研究为红豆杉属植物JA调控系统的研究以及阐明JA信号通路对陆地植物的意义提供了依据。
