视网膜中肌醇-1,4,5-三磷酸激酶的检测。ATP对肌醇-1,4,5-三磷酸磷酸化的直接证明。
Detection of inositol 1,4,5-trisphosphate kinase in retina. A direct demonstration of phosphorylation of inositol 1,4,5-trisphosphate by ATP.
作者信息
Tarver A P, Anderson R E
机构信息
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
出版信息
Biochem J. 1988 Mar 15;250(3):891-5. doi: 10.1042/bj2500891.
The metabolism of myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] consists of two pathways: dephosphorylation by 5-phosphomonoesterase(s) produces inositol 1,4-bisphosphate, and phosphorylation by Ins(1,4,5)P3 3-kinase yields inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. The requirements for Ins(1,4,5)P3 kinase activity in retina were characterized. Apparent Km values for ATP and Ins(1,4,5)P3 are 1.4 mM and 1.3 microM respectively. A direct demonstration of phosphorylation of Ins(1,4,5)P3 by [gamma-32P]ATP was achieved. Characterization of the 32P-labelled product revealed that it had the expected chromatographic and electrophoretic properties of Ins(1,3,4,5)P4.
肌醇1,4,5-三磷酸[Ins(1,4,5)P3]的代谢有两条途径：5-磷酸单酯酶使其去磷酸化产生肌醇1,4-二磷酸，而Ins(1,4,5)P3 3-激酶使其磷酸化生成肌醇1,3,4,5-四磷酸[Ins(1,3,4,5)P4]。对视网膜中Ins(1,4,5)P3激酶活性的要求进行了表征。ATP和Ins(1,4,5)P3的表观Km值分别为1.4 mM和1.3 microM。通过[γ-32P]ATP实现了对Ins(1,4,5)P3磷酸化的直接证明。对32P标记产物的表征显示，它具有Ins(1,3,4,5)P4预期的色谱和电泳性质。
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