巯基试剂对猪脑微粒体中二十六碳烯酸合成的激活作用。

Activation of synthesis of hexacosenoic acid by sulfhydryl reagents in swine cerebral microsomes.

作者信息

Saitoh T, Yoshida S, Takeshita M

机构信息

Department of Biochemistry, Medical College of Oita, Japan.

出版信息

Biochem Int. 1988 Apr;16(4):671-8.

PMID:2839189

Abstract

The elongation of icosenoyl-CoA (20:1-CoA) in swine cerebral microsomes resulted in the synthesis of docosenoic acid (22:1) and tetracosenoic acid (24:1), but the synthesis of hexacosenoic acid (26:1) was negligible. In contrast, in the presence of sulfhydryl reagents (0.6 mM N-ethylmaleimide [NEM] or 0.3 mM p-chloromercuriphenylsulfonic acid [PCMPS]) the synthesis of 26:1 was remarkably enhanced. We suggest that the synthesis of 26:1 from 20:1-CoA was more enhanced by NEM or PCMPS as a result of activation of the condensation step in the elongation of 24:1 (intermediate) to 26:1.

摘要

猪脑微粒体中二十碳烯酰辅酶A（20:1-CoA）的延长反应生成了二十二碳烯酸（22:1）和二十四碳烯酸（24:1），但二十六碳烯酸（26:1）的合成量可忽略不计。相比之下，在巯基试剂（0.6 mM N-乙基马来酰亚胺[NEM]或0.3 mM对氯汞苯磺酸[PCMPS]）存在的情况下，26:1的合成显著增强。我们认为，NEM或PCMPS通过激活24:1（中间体）延长至26:1过程中的缩合步骤，从而更显著地促进了由20:1-CoA合成26:1。