Costas M J, Cameselle J C
Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
Biochem Int. 1988 Apr;16(4):747-53.
The sensitivity of the Mg(II)-dependent activity of rabbit liver fructose 1,6-bisphosphatase (FBPase, EC 3.1.3.11) to inhibition by fructose 2,6-bisphosphate (Fru-2,6-P2) was enhanced by EDTA and diminished to negligible levels by 0.5-2 microM Zn(II) added as another FBPase inhibitor. Fru-2,6-P2 was more efficient in the presence of the synergistic effector AMP: still, the Fru-2,6-P2 concentration inhibiting 50% changed from 3 microM (with EDTA) to higher than 50 microM (with Zn(II]. On the other hand, the Zn(II)-dependent FBPase activity was inhibited by Fru-2,6-P2 to a much lesser extent than the Mg(II)-dependent activity.
兔肝果糖1,6 - 二磷酸酶（FBPase，EC 3.1.3.11）的镁离子依赖性活性对果糖2,6 - 二磷酸（Fru-2,6-P2）抑制作用的敏感性在加入乙二胺四乙酸（EDTA）后增强，而当加入另一种FBPase抑制剂0.5 - 2微摩尔锌离子（Zn(II)）时，该敏感性降低至可忽略不计的水平。在协同效应物腺苷一磷酸（AMP）存在的情况下，Fru-2,6-P2的作用更有效：然而，抑制50%活性的Fru-2,6-P2浓度从3微摩尔（加入EDTA时）变为高于50微摩尔（加入Zn(II)时）。另一方面，Fru-2,6-P2对锌离子依赖性FBPase活性的抑制程度远小于对镁离子依赖性活性的抑制程度。
