Tsuda K, Tsuda S, Minatogawa Y, Shima H, Yoshikawa H, Nishio I, Kido R, Masuyama Y
Department of Medicine, Wakayama Medical College, Japan.
Am J Hypertens. 1988 Jul;1(3 Pt 1):283-6. doi: 10.1093/ajh/1.3.283.
We examined alterations in membrane fluidity of hypertension and the effects of calcium antagonists on these changes by means of an electron spin resonance (ESR) and spin-label technique. Washed erythrocytes from spontaneously hypertensive rats (SHRs; aged 4 and 10-13 weeks) and from patients with essential hypertension, or cultured vascular smooth muscle cells from SHRs were examined and compared with those from normotensive controls. Electron spin resonance spectra for a fatty acid spin label-agent (5-nitroxy stearate) in the membranes were obtained. The values of hyperfine splitting and other parameters of the spectra were significantly higher in erythrocytes from hypertensive rats than in normotensive controls. Similar results were obtained in cultured vascular smooth muscles. These findings indicate that the membrane fluidity might be decreased in hypertension. When calcium was loaded to erythrocytes with Ca-ionophore A23187, the fluidity was decreased. The alternative degrees were greater in hypertensive rats than in controls, and these hypertensive changes were antagonized by diltiazem or verapamil. The results revealed that abnormalities of membrane fluidity in hypertension might be more prominent in the presence of calcium. Further, it is suggested that calcium antagonists could correct these membrane abnormalities in hypertension.
我们通过电子自旋共振（ESR）和自旋标记技术，研究了高血压患者膜流动性的改变以及钙拮抗剂对这些变化的影响。检测并比较了自发性高血压大鼠（SHR，4周龄和10 - 13周龄）、原发性高血压患者的洗涤红细胞，或SHR的培养血管平滑肌细胞与正常血压对照者的相应细胞。获得了膜中脂肪酸自旋标记剂（5 - 硝基硬脂酸盐）的电子自旋共振光谱。高血压大鼠红细胞的超精细分裂值和光谱的其他参数显著高于正常血压对照者。在培养的血管平滑肌中也得到了类似结果。这些发现表明高血压患者的膜流动性可能降低。当用钙离子载体A23187使红细胞加载钙离子时，流动性降低。高血压大鼠的变化程度大于对照组，并且这些高血压相关变化可被地尔硫䓬或维拉帕米拮抗。结果显示，在有钙存在的情况下，高血压患者膜流动性异常可能更明显。此外，提示钙拮抗剂可纠正高血压患者的这些膜异常。
