Sun Jing, Zhang Xin, Sun Yang, Tang Zhi-Shu, Guo Dong-Yan
College of Pharmacy, Shannxi University of Chinese Medicine, Xianyang, Shannxi 712046, P.R. China.
Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an, Shannxi 710032, P.R. China.
Mol Med Rep. 2017 Jun;15(6):3485-3492. doi: 10.3892/mmr.2017.6426. Epub 2017 Apr 3.
Hylomecon vernalis Maxim. has traditionally been used to promote blood circulation, alleviate pain, dissipate stasis, and reduce swelling. The aim of the present study was to investigate the effect and potential mechanism of H. vernalis Maxim. ethanol extracts (HVMEE) on the growth and apoptosis of human colon cancer HT‑29 and SW620 cells. H. vernalis samples were extracted three times with ethanol, dried, and concentrated into powder. The components of HVMEE were investigated using high performance liquid chromatography in tandem with mass spectrometry analysis. MTT assay was used to investigate the effect of HVMEE on viability of human colon cancer HT‑29 and SW620 cells. Apoptosis of HT‑29 and SW620 cells was evaluated using flow cytometric analysis. Expression levels of apoptosis and cell cycle‑related proteins were assessed by western blot. The findings demonstrated that the alkaloid content of HVMEE was as high as 89.67%, and it effectively inhibited viability in HT‑29 and SW620 cells, with IC50 values of 0.105±0.022 mg/ml and 0.146±0.013 mg/ml, respectively. In addition, HVMEE induced apoptosis in HT‑29 and SW620 cells, by increasing caspase‑3, caspase‑9 and BCL2 associated X expression, and reducing Bcl‑2 expression. The present study suggests that HVMEE has a potential role in the treatment of colorectal cancer.
延胡索乙素传统上被用于促进血液循环、缓解疼痛、消散瘀血和减轻肿胀。本研究的目的是探讨延胡索乙素乙醇提取物（HVMEE）对人结肠癌HT-29和SW620细胞生长和凋亡的影响及潜在机制。将延胡索样本用乙醇提取三次，干燥并浓缩成粉末。采用高效液相色谱串联质谱分析法对HVMEE的成分进行研究。采用MTT法研究HVMEE对人结肠癌HT-29和SW620细胞活力的影响。采用流式细胞术分析评估HT-29和SW620细胞的凋亡情况。通过蛋白质印迹法评估凋亡和细胞周期相关蛋白的表达水平。结果表明，HVMEE中生物碱含量高达89.67%，它能有效抑制HT-29和SW620细胞的活力，IC50值分别为0.105±0.022mg/ml和0.146±0.013mg/ml。此外，HVMEE通过增加半胱天冬酶-3、半胱天冬酶-9和BCL2相关X蛋白的表达，并降低Bcl-2蛋白的表达，诱导HT-29和SW620细胞凋亡。本研究表明，HVMEE在结直肠癌治疗中具有潜在作用。
