Zhou Jingzhu, Li Shiying, Wang Yinping, Foreman Robert D, Yin Jieyun, Zhang Shengsheng, Chen Jiande D Z
Veterans Research and Education Foundation, VA Medical Center, Oklahoma City, OK, USA.
Department of Physiology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA.
Neuromodulation. 2017 Dec;20(8):767-773. doi: 10.1111/ner.12602. Epub 2017 Apr 10.
Stress is considered an independent factor causing and aggravating gastrointestinal symptoms, including visceral pain. The aim of this study was to investigate effects and mechanisms of electroacupuncture (EA) on stress-induced gastric hypersensitivity in rats treated with neonatal iodoacetamide mimicking human functional dyspepsia (FD).
Neonatal rats were treated with gavage of 0.2 mL of 0.1% iodoacetamide in 2% sucrose daily for six days starting on tenth day after birth. The control group was given 0.2 mL of 2% sucrose. When the rats were eight weeks old, acute restraint stress was performed on them for 90 min. EA at ST36 (ZuSanLi) was performed during the acute stress or 30 min after the stress. Adrenoceptor blocking drugs (propranolol and phentolamine) were injected intraperitoneally 30 min before acute restraint stress to explore possible sympathetic mechanisms. Visceral-motor responses to gastric distention were assessed by electromyogram (EMG).
Rats treated with neonatal iodoacetamide mimicking FD are more vulnerable to stress. Stress-induced gastric hypersensitivity can be prevented or suppressed by EA at ST36 via the restoration of sympathovagal balance.
应激被认为是导致和加重胃肠道症状（包括内脏疼痛）的一个独立因素。本研究旨在探讨电针（EA）对用新生期碘乙酰胺处理以模拟人类功能性消化不良（FD）的大鼠应激诱导的胃超敏反应的影响及机制。
新生大鼠从出生后第10天开始，每天用灌胃法给予0.2 mL含0.1%碘乙酰胺的2%蔗糖溶液，持续6天。对照组给予0.2 mL 2%蔗糖溶液。当大鼠8周龄时，对其进行90分钟的急性束缚应激。在急性应激期间或应激后30分钟进行足三里（ST36）电针治疗。在急性束缚应激前30分钟腹腔注射肾上腺素能受体阻断药物（普萘洛尔和酚妥拉明），以探究可能的交感神经机制。通过肌电图（EMG）评估对胃扩张的内脏运动反应。
1）与对照大鼠相比，FD大鼠应激诱导的胃超敏反应明显更严重。它被肾上腺素能受体拮抗剂阻断。2）电针抑制应激诱导的胃超敏反应；电针的预防作用（在应激期间给予）比治疗作用（在应激后给予）更显著。应激导致更高的交感迷走神经比值，而电针可抑制这一比值。
用新生期碘乙酰胺处理以模拟FD的大鼠对应激更敏感。电针足三里可通过恢复交感迷走神经平衡预防或抑制应激诱导的胃超敏反应。
