Yuan Quan, Sun Li, Yu Honghao, An Chunhou
a Department of Orthopedics , Shengjing Hospital of China Medical University , Shenyang , People's Republic of China.
b Department of Nephrology , The First Affiliated Hospital of China Medical University , Shenyang , People's Republic of China.
Biosci Biotechnol Biochem. 2017 Jul;81(7):1335-1342. doi: 10.1080/09168451.2017.1313695. Epub 2017 Apr 10.
Our previous study found that co-culture with human vascular endothelial cells (HMVECs) is beneficial for dorsal root ganglion cells (DRGCs). The goal of the present study is to investigate whether co-culture with HMVECs could promote the development of DRGCs, and whether this effect is induced by the secretion of BDNF by HMVECs. DRGCs were mono-cultured, co-cultured with HMVECs or co-cultured with HMVECs that pre-transfected with BDNF siRNA, the expression of neurite formation and branching factors were determined. The results showed that transfecting with BDNF siRNA inhibited BDNF expression and reduced BDNF secretion. Co-culture with HMVECs increased the expression of Etv4, Etv5, FN-L, FN-M, and GAP-43 in DRGCs that accompanied by the activation of ERK pathway. However, these changes were all reversed by the inhibition of BDNF in HMVECs. In conclusion, our data demonstrate that HMVECs potentiated DRGCs development at least partly by the secretion of BDNF in the co-culture system.
我们之前的研究发现，与人类血管内皮细胞（HMVECs）共培养对背根神经节细胞（DRGCs）有益。本研究的目的是调查与HMVECs共培养是否能促进DRGCs的发育，以及这种效应是否由HMVECs分泌的脑源性神经营养因子（BDNF）所诱导。将DRGCs进行单培养、与HMVECs共培养或与预先转染了BDNF小干扰RNA（siRNA）的HMVECs共培养，然后测定神经突形成和分支因子的表达。结果显示，转染BDNF siRNA可抑制BDNF表达并减少BDNF分泌。与HMVECs共培养可增加DRGCs中Etv4、Etv5、FN-L、FN-M和GAP-43的表达，同时伴有细胞外信号调节激酶（ERK）通路的激活。然而，HMVECs中BDNF的抑制可逆转这些变化。总之，我们的数据表明，在共培养系统中，HMVECs至少部分地通过分泌BDNF来增强DRGCs的发育。
