用针对主要病毒核心蛋白p26的单克隆抗体鉴定马传染性贫血病毒的gag前体。

Identification of gag precursor of equine infectious anaemia virus with monoclonal antibodies to the major viral core protein, p26.

作者信息

Hussain K A, Issel C J, Rwambo P M, Arnizaut A B, Ball J M, Schnorr K L, Montelaro R C

机构信息

Department of Veterinary Microbiology and Parasitology, Louisiana State University School of Veterinary Medicine, Baton Rouge.

出版信息

J Gen Virol. 1988 Jul;69 ( Pt 7):1719-24. doi: 10.1099/0022-1317-69-7-1719.

DOI:10.1099/0022-1317-69-7-1719

PMID:2839604

Abstract

Monoclonal antibodies (MAbs) against the major core protein p26 of equine infectious anaemia virus (EIAV) were produced and characterized. Sensitive enzyme-linked immunosorbent assay and Western blot immunoassay were employed to confirm the specificity of these MAbs. Western blot analysis also indicated that MAbs to p26 reacted with another EIAV protein of 55,000 apparent Mr (designated here as Pr55gag) present in density gradient-purified virus preparations. Rabbit antiserum prepared against p26 as well as MAbs to p26 detected Pr55gag and several other intermediate clevage products in detergent-soluble lysates of virus-infected cells in Western blot and immunoprecipitation assays. The results suggest that Pr55gag is the gag polyprotein of EIAV.

摘要

制备并鉴定了抗马传染性贫血病毒（EIAV）主要核心蛋白p26的单克隆抗体（MAb）。采用灵敏的酶联免疫吸附测定和蛋白质印迹免疫测定来确认这些单克隆抗体的特异性。蛋白质印迹分析还表明，针对p26的单克隆抗体与存在于密度梯度纯化病毒制剂中的另一种表观分子量为55,000的EIAV蛋白（在此称为Pr55gag）发生反应。在蛋白质印迹和免疫沉淀试验中，针对p26制备的兔抗血清以及针对p26的单克隆抗体在病毒感染细胞的去污剂可溶裂解物中检测到Pr55gag和其他几种中间裂解产物。结果表明，Pr55gag是EIAV的gag多蛋白。